Enzymatic modular synthesis and microarray assay of poly-N-acetyllactosamine derivatives. Issue 55 (24th June 2020)

• Record Type: Journal Article
• Authors: Chen, Congcong; Wang, Shuaishuai; Gadi, Madhusudhan Reddy; Zhu, He; Liu, Fei; Liu, Chang-Cheng; Li, Lei; Wang, Fengshan; Ling, Peixue; Cao, Hongzhi
• Is Part Of: Chemical communications. Volume 56:Issue 55(2020)
• Page Start: 7549
• Page End: 7552
• Publication Date: 2020-06-24
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d0cc03268a
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : An efficient enzymatic modular assembly strategy was developed for the preparative-scale synthesis of poly-LacNAc glycans with defined fucosylation and sialylation patterns. Abstract : A facile enzymatic modular assembly strategy for the preparative-scale synthesis of poly- N -acetyllactosamine (poly-LacNAc) glycans with varied lengths and designed sialylation and/or fucosylation patterns is described. These glycans were printed as a microarray to investigate their interactions with a panel of glycan binding proteins (GBPs). Binding affinities revealed that the avidity of GBPs could be largely affected by the length and the patterns of sialylation and fucosylation.