Enzymatic modular synthesis and microarray assay of poly-N-acetyllactosamine derivatives. Issue 55 (24th June 2020) - Chen, Congcong
Abstract : An efficient enzymatic modular assembly strategy was developed for the preparative-scale synthesis of poly-LacNAc glycans with defined fucosylation and sialylation patterns. Abstract : A facile enzymatic modular assembly strategy for the preparative-scale synthesis of poly- N -acetyllactosamine (poly-LacNAc) glycans with varied lengths and designed sialylation and/or fucosylation patterns is described. These glycans were printed as a microarray to investigate their interactions with a panel of glycan binding proteins (GBPs). Binding affinities revealed that the avidity of GBPs could be largely affected by the length and the patterns of sialylation and fucosylation.