A novel endogenous antimicrobial peptide CAMP211-225 derived from casein in human milk. Issue 3 (27th February 2020)

• Record Type: Journal Article
• Authors: Wang, Xing; Sun, Yazhou; Wang, Fei; You, Lianghui; Cao, Yan; Tang, Ranran; Wen, Juan; Cui, Xianwei
• Is Part Of: Food & function. Volume 11:Issue 3(2020)
• Page Start: 2291
• Page End: 2298
• Publication Date: 2020-02-27
• Subjects: Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Nutrition -- Periodicals; 664.07
• DOI: 10.1039/c9fo02813g
• Languages: English
• ISSNs: 2042-6496

Abstract

Abstract : A novel endogenous peptide CAMP211-225 up-regulated in preterm milk specifically kills E. coli and Y. enterocolitica through a membrane-disrupting mechanism. Abstract : A large number of bioactive peptides derived from breast milk have been identified to be multifunctional having anti-inflammatory, immunoregulatory and antimicrobial activities. Here, we report that an endogenous peptide located at β-casein 211-225 amino acid from human breast milk (hereafter called CAMP211-225 ) presents specific antimicrobial activity against pathogenic E. coli and Y. enterocolitica . CAMP211-225 is a novel peptide that occurs at higher levels in preterm milk than in term milk. The minimal inhibitory concentrations (MIC) of CAMP211-225 against E. coli and Y. enterocolitica are 3.125 μg ml −1 and 6.25 μg ml −1, respectively, and the antimicrobial activity of CAMP211-225 was also confirmed by a disk diffusion assay. Further studies using fluorescence staining, scanning electron microscopy and a DNA-binding assay revealed that CAMP211-225 kills bacteria through a membrane-disrupting mechanism, but not by binding to intracellular nucleic acids. Neonatal necrotizing enterocolitis (NEC) is a devastating gastrointestinal disease in neonatal intensive care units. In our study, CAMP211-225 administration effectively reduced ileal mucosa damage in an experimental NEC mice model. These results suggest that the antimicrobial peptide CAMP211-225 may have potential value in the prevention and treatment of neonatal infections.