Characterization of bioactive compounds in defatted hempseed (Cannabis sativa L.) by UHPLC-HRMS/MS and anti-inflammatory activity in primary human monocytes. Issue 5 (24th April 2020)

• Record Type: Journal Article
• Authors: Rea Martinez, Julio; Montserrat-de la Paz, Sergio; De la Puerta, Rocío; García-Giménez, M. Dolores; Fernández-Arche, M. Ángeles
• Is Part Of: Food & function. Volume 11:Issue 5(2020)
• Page Start: 4057
• Page End: 4066
• Publication Date: 2020-04-24
• Subjects: Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Nutrition -- Periodicals; 664.07
• DOI: 10.1039/d0fo00066c
• Languages: English
• ISSNs: 2042-6496

Abstract

Abstract : Anti-inflammatory effects on human monocytes by phenolic amides present in defatted hempseed fractions ( Cannabis sativa L.). Abstract : Hempseed ( Cannabis sativa L.) has beneficial impact on human health mainly because of its wide variability of bioactive compounds. However, many of them are not fully characterized yet. In this work, hempseed was defatted and through a bio-guided studied, two fractions (F03 and F05) with the highest content of phenols, flavonoids and antioxidant capacity were selected. Fractions were chemically analyzed by UHPLC HRMS/MS. The anti-inflammatory capacities of these compounds were evaluated on human monocytes using flow cytometry, RT-qPCR and Elisa procedures. A high amount of phenolic compounds were identified, with the major compound being: N-trans -caffeoyltyramine (6.36 mg g −1 in F05 and 1.28 mg g −1 in F03). Both, F03 and F05 significantly reduced the inflammatory competence of LPS-treated human primary monocytes, decreasing TNF-α and IL-6 gene expression and secretion. These findings indicate that in the defatted fraction of the hempseed there are a wide number of compounds with beneficial potential to prevent and treat inflammatory disorders, as well as other processes caused by oxidative stress.