Secretome analysis of thermophilic mould Myceliophthora thermophila cultivated on rice straw and hydrolysis of lignocellulosic biomass for bioethanol production. Issue 4 (3rd July 2020)

• Record Type: Journal Article
• Authors: Bala, Anju; , Anu; , Alokika; Kumar, Anil; Kumar, Sanjeev; Singh, Davender; Singh, Bijender
• Is Part Of: Biocatalysis and biotransformation. Volume 38:Issue 4(2020)
• Page Start: 283
• Page End: 292
• Publication Date: 2020-07-03
• Subjects: Myceliophthora thermophila BJAMDU5 -- secretome -- CAZymes -- rice straw -- bioethanol; Enzymes -- Biotechnology -- Periodicals; Enzymes -- Industrial applications -- Periodicals; Biotransformation (Metabolism) -- Periodicals; 660.63
• DOI: 10.1080/10242422.2020.1711743
• Languages: English
• ISSNs: 1024-2422

Abstract

Abstract: Myceliophthora thermophila encodes for large number of carbohydrate-active enzymes (CAZymes) involved in lignocellulosic biomass degradation. The mould was grown on rice straw in solid state fermentation at pH 5.0 and 45 °C that produced high levels of cellulolytic and xylanolytic enzymes i.e. 2218.12, 515.23, 478.23, 13.34 U/g DMR for xylanase, CMCase, FPase and β-glucosidase, respectively. The secretome analysis of M. thermophila BJAMDU5 by mass spectroscopy, described 124 different proteins with majority of CAZymes consisting of glycosyl hydrolases (GH), lytic polysaccharide mono-oxygenases (LPMO), carbohydrate esterases (CE) and polysaccharide lyases (PL). Furthermore, the enzyme cocktail of the mould was evaluated for hydrolysis of steam treated rice straw that produced 184.59 mg/g substrate reducing sugars after 24 h, which was used for production of bioethanol by using fast fermenting yeast Saccharomyces cerevisiae resulting in high production of bioethanol. Graphical Abstract: