Polyphenol composition and biological activity of Thymus citriodorus and Thymus vulgaris: Comparison with endemic Iberian Thymus species. (30th November 2020)

• Record Type: Journal Article
• Authors: Taghouti, Meriem; Martins-Gomes, Carlos; Félix, Luís M.; Schäfer, Judith; Santos, João A.; Bunzel, Mirko; Nunes, Fernando M.; Silva, Amélia M.
• Is Part Of: Food chemistry. Volume 331(2020)
• Publication Date: 2020-11-30
• Subjects: Apigenin (PubChem CID: 5280443) -- Caffeic acid (PubChem CID: 689043) -- Chrysoeriol (Pubchem CID 5280666) -- Eriodictyol (Pubchem CID 440735) -- Luteolin (PubChem CID: 5280445) -- Rosmarinic acid (PubChem CID: 5281792) -- Salvianolic acid A (PubChem CID: 5281793) -- Salvianolic acid I (Pubchem CID 10459878) -- Salvianolic acid K (Pubchem CID 10482829); Thymus L. species -- Thymus × citriodorus -- Thymus vulgaris -- Phenolic compound profiling -- Antioxidant activity -- Anti-proliferative activity -- Caco-2 -- HepG2; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2020.127362
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: T. citriodorus and T. vugaris phenolic profile is described and compared. Rosmarinic acid is the major phenolic in T. citriodorus and T. vugaris extracts. Both extracts showed significant radical scavenging activity. T. citriodorus phenol profile was more similar to T. vulgaris than to T. pulegioides. Salvianolic acids levels in Thymus is associated with extracts bioactivity. Abstract: The polyphenol compositions of Thymus × citriodorus and Thymus vulgaris extracts as obtained by exhaustive hydroethanolic (HE) extraction and aqueous decoction (AD) were compared. In addition, their compositions and bioactivities were compared to those of Thymus pulegioides and Thymus mastichina, grown under the same edaphoclimatic conditions, and Thymus carnosus . Rosmarinic acid was the most abundant polyphenol followed by luteolin-hexuronide, salvianolic acids I and K. Cluster analysis suggests a similarity of the polyphenol composition of T. citriodorus and T. vulgaris . A significant antioxidant activity was observed and correlated with their polyphenol levels. The same being observed for the higher anti-proliferative activity/cytotoxicity of HE extracts on Caco-2 and HepG2 cells as compared to AD extracts. Significant association between the total phenolic compounds with the anti-proliferative activity, for both cell lines, was observed. These results support the importance of salvianolic acids levels in Thymus extracts and their in vitro anti-proliferative/cytotoxic activities.