Lipase catalysis of α‐linolenic acid‐rich medium‐ and long‐chain triacylglycerols from perilla oil and medium‐chain triacylglycerols with reduced by‐products. (24th June 2020)

• Record Type: Journal Article
• Authors: Huang, Zhaoxian; Cao, Zhenyu; Guo, Zengwang; Chen, Liang; Wang, Zhongjiang; Sui, Xiaonan; Jiang, Lianzhou
• Is Part Of: Journal of the science of food and agriculture. Volume 100:Number 12(2020)
• Page Start: 4565
• Page End: 4574
• Publication Date: 2020-06-24
• Subjects: medium‐ and long‐chain triacylglycerols -- α‐linolenic -- enzymatic interesterification -- perilla oil -- two‐stage deodorization; Food -- Periodicals; Agriculture -- Periodicals; 664
• DOI: 10.1002/jsfa.10515
• Languages: English
• ISSNs: 0022-5142

Abstract

Abstract: BACKGROUND: Medium‐ and long‐ chain triacylglycerols (MLCTs) are functional structural lipids that can provide the human body with essential fatty acids and a faster energy supply. This study aimed to prepare MLCTs rich in α ‐linolenic by enzymatic interesterification of perilla oil and medium‐chain triacylglycerols (MCTs), catalyzed by Lipozyme RM IM, Lipozyme TL IM, Lipozyme 435, and Novozyme 435 respectively. RESULTS: The effects of lipase loading, concentration of MCTs, reaction temperature, and reaction time on the yield of MLCTs were investigated. It was found that the reaction achieved more than a 70% yield of MLCTs in triacylglycerols under the conditions of 400 g kg −1 MCTs and 60 g kg −1 lipase loading after equilibrium. A novel two‐stage deodorization was also applied to purify the interesterification products. The triacylglycerols reach over 97% purity in the products with significant removal ( P < 0.05) of the free fatty acids, and the trans fatty acids were strictly controlled at below 1%. There was more than 40% α ‐linolenic in the purified products, with long‐chain fatty acids mostly occupying the desired sn ‐2 position in acylglycerols, which are more active in hydrolysis. CONCLUSION: A series of novel α ‐linolenic acid‐rich medium‐ and long‐chain triacylglycerols was prepared. Under appropriate reaction conditions, the yield of MLCTs in triacylglycerols was above 70%. A novel two‐stage deodorization can be used to promote the elimination of free fatty acids and limit the generation of trans fatty acids. © 2020 Society of Chemical Industry