Preparation and characterization of amino and carboxyl functionalized core-shell Fe3O4/SiO2 for L-asparaginase immobilization: A comparison study. Issue 5 (2nd September 2020)

• Record Type: Journal Article
• Authors: Noma, Samir Abbas Ali; Ulu, Ahmet; Koytepe, Suleyman; Ateş, Burhan
• Is Part Of: Biocatalysis and biotransformation. Volume 38:Issue 5(2020)
• Page Start: 392
• Page End: 404
• Publication Date: 2020-09-02
• Subjects: Magnetic nanoparticles -- silica coating -- L-asparaginase -- enzyme immobilization -- enhanced stability; Enzymes -- Biotechnology -- Periodicals; Enzymes -- Industrial applications -- Periodicals; Biotransformation (Metabolism) -- Periodicals; 660.63
• DOI: 10.1080/10242422.2020.1767605
• Languages: English
• ISSNs: 1024-2422

Abstract

Abstract: Magnetic nanoparticles are well known as facile and effective support for enzyme immobilization since they have a high surface area, large surface-to-volume ratio, easy separation, a fast and high enzyme loading. This study aims to provide insights on whether acidic or basic modified particles are more effective for L-asparaginase (ASNase) immobilization. Therefore, amino (Fe3 O4 /SiO2 /NH2 ) and carboxyl-functionalized (Fe3 O4 /SiO2 /COOH) particles were prepared. The functional groups, crystalline structure, magnetic properties, morphology, chemical composition and thermal behaviour of the prepared modified nanoparticles were examined via Fourier-transform infra-red spectroscopy (FTIR), X-ray diffraction (XRD), vibrating-sample magnetometer (VSM), scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDAX). Under the optimum conditions, the immobilized enzymes were more stable within a certain range of temperatures and pH values in comparison to free enzyme. On the other hand, the immobilized enzymes showed greater stability after incubation for 3 h at 50 °C. The free enzyme maintained only 30% of its initial activity for 4 weeks at 4 °C, while Fe3 O4 /SiO2 /NH2 /ASNase and Fe3 O4 /SiO2 /COOH/ASNase retained more than 78.9% and 56.5% of initial activities under the same conditions, respectively. Moreover, Fe3 O4 /SiO2 /NH2 /ASNase (77.2%) and Fe3 O4 /SiO2 /COOH/ASNase (57.4%) displayed excellent operational stability after 17 repeated cycles. These findings suggested that the Fe3 O4 /SiO2 /NH2 and Fe3 O4 /SiO2 /COOH may be utilized as efficient and sustainable supports to developed immobilized ASNase in several biotechnological applications.