An innovative paper-based device for DNA extraction from processed meat products. (15th August 2020)

• Record Type: Journal Article
• Authors: Batule, Bhagwan S.; Seok, Youngung; Kim, Min-Gon
• Is Part Of: Food chemistry. Volume 321(2020)
• Publication Date: 2020-08-15
• Subjects: Paper chip -- DNA extraction -- Mitochondrial DNA -- PCR analysis -- Meat detection; Tris hydrochloride (PubChem CID 93573) -- Guanidinium thiocyanate (PubChem CID 129830882) -- Dithiothreitol (PubChem CID 446094); Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2020.126708
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: One-step rapid (~5 min) extraction of mtDNA from processed meat products. Sensitive and selective detection of meat types up to 0.1% adulteration. Successfully identified meat types from commercially available meat products. Abstract: Detection of food adulteration is a challenge. However, the identification of adulterated meat in processed products is important for health and personal preference. Mitochondrial genomic DNA (mtDNA) is a good candidate for reliable identification of meat ingredients; however, the extraction of mtDNA from processed products is a bottleneck for development of detection strategies. Therefore, we constructed a rapid (~5 min) mtDNA extraction device. mtDNAs from different meat samples, such as pork ( Sus scrofa ), chicken ( Gallus gallus ), and beef ( Bos taurus ), were successfully detected in up to 0.1% adulterated animal species. We believe that the proposed strategy could be applied to detect animal species from processed meat products to reduce fraudulent practices.