An exploratory study on the simultaneous screening for residues of chloramphenicol, ciprofloxacin and sulphadimidine using recombinant antibodies. Issue 5 (3rd May 2020)

• Record Type: Journal Article
• Authors: Wang, Ying; An, Yu; Liu, Zengshan; Zhou, Yu; Zhang, Dongjie
• Is Part Of: Food additives & contaminants. Volume 37:Issue 5(2020)
• Page Start: 763
• Page End: 769
• Publication Date: 2020-05-03
• Subjects: Single chain antibody -- gene link -- prokaryotic expression -- antibiotics -- screening -- ELISA; Food additives -- Periodicals; Food contamination -- Periodicals; 664.06
• DOI: 10.1080/19440049.2019.1591641
• Languages: English
• ISSNs: 1944-0049

Abstract

ABSTRACT: A recombinant gene CCS consisting of three single genes (CAP-ScFv, CPFX-ScFv and SM2 -ScFv) were constructed by PCR with three pairs of primers. The length of CCS gene fragment was 2, 260 bp. A recombinant plasmid (pGEX-CCS) was obtained in pGEX-6p-1. pGEX-CCS was induced in E.coli BL21(DE3), and the molecular weight of recombinant fusion protein (GST-CCS) was 108.87 kDa. GST-CCS was successfully applied to analysis of CAP-OVA (CAP-ovalbumin conjugate), CPFX-OVA and SM2 -OVA simultaneously. The sensitivity of GST-CCS against three veterinary drugs was tested by indirect ELISA at dilution ratio 1:8000. These findings provide a foundation for the construction of fusion genes with linkers and for the potential development of a rapid screening method for the simultaneous detection of veterinary drug residues.