Effects of conventional and nonconventional drying on the stability of Bifidobacterium animalis subsp. lactis INL1. (9th January 2020)

• Record Type: Journal Article
• Authors: Zacarías, María Florencia; Reinheimer, Jorge A; Vinderola, Gabriel; Kulozik, Ulrich; Ambros, Sabine
• Is Part Of: International journal of dairy technology. Volume 73:Number 3(2020)
• Page Start: 625
• Page End: 633
• Publication Date: 2020-01-09
• Subjects: Bifidobacteria -- Drying -- Membrane integrity -- Probiotics -- Storage -- Survival; Dairying -- Periodicals; Dairying -- Great Britain -- Periodicals; 637
• DOI: 10.1111/1471-0307.12684
• Languages: English
• ISSNs: 1364-727X

Abstract

Abstract : Freeze, spray, vacuum, microwave vacuum‐ and microwave freeze‐drying were applied to Bifidobacterium animalis subsp. lactis INL1. Freeze and microwave freeze‐drying showed the highest survival after drying. When a storage test (25 °C; oxygen) was performed, these cultures were the most sensitive ones at a w = 0.23, but the addition of lactose improved their stability after 8 weeks. Flow cytometry was useful to assess viability after drying but not during storage. Our results show that dehydration technologies other than freeze‐drying might be suitable alternatives that deserve further investigation for the preservation of sensitive probiotic bacteria. Microwave drying rendered cultures of comparable characteristics to their conventional counterparts, requiring significantly shorter drying times.