Unveiling the anticancer and antimycobacterial potentials of bioengineered gold nanoparticles. (September 2020)

• Record Type: Journal Article
• Authors: Govindaraju, K.; Vasantharaja, R.; Uma Suganya, K.S.; Anbarasu, S.; Revathy, K.; Pugazhendhi, A.; Karthickeyan, D.; Singaravelu, G.
• Is Part Of: Process biochemistry. Volume 96(2020)
• Page Start: 213
• Page End: 219
• Publication Date: 2020-09
• Subjects: Gold nanoparticles -- Anticancer -- Toxicity -- Antimycobacterial activity -- Pongammia pinnata; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2020.06.016
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Preparation of bioengineered gold nanoparticles using Pongammia pinnata . Anticancer activity of bioengineered gold nanoparticles in breast cancer cell line. Antimycobacterial activity of bioengineered gold nanoparticles. Abstract: Synthesis of gold nanoparticles was carried out using Pongammia pinnata (pongam) leaf extract and their anticancer and antimycobacterial activities were studied. Gold nanoparticle formation was confirmed by UV–vis, XRD and HR-TEM. The anticancer efficacies of the biogenic gold nanoparticles were analyzed using cytotoxicity, cell morphology analysis, oxidative DNA damage, apoptosis detection and toxicity studies. Biogenic gold nanoparticles inhibited breast cancer cell line (MCF-7) proliferation with an efficacy of IC50 of 1.85 μg/mL. The antimycobacterial potential of the biogenic gold nanoparticles was screened against M. tuberculosis by Luciferase Reporter Phage (LRP) assay. The gold nanoparticles showed inhibition against sensitive M. tuberculosis with the minimum inhibitory concentration (MIC) of 10 μg/mL whereas no inhibition was found against the rifampicin resistant M. tuberculosis.