Mechanism of Ca2+-mediated NOX modulated in ROS metabolism induced by T-2 toxin in potato tuber. (1st July 2020)

• Record Type: Journal Article
• Authors: Rui, Zhang; Huali, Xue; Min, Si; Yang, Bi; Mina, Nan; Yuanyuan, Zong; Haitao, Long; Prusky, Dov; Xiaoyan, Cheng
• Is Part Of: Food chemistry. Volume 317(2020)
• Publication Date: 2020-07-01
• Subjects: Potato tuber -- EGTA -- Ca2+ -- NADPH oxidase -- Reactive oxygen species; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2020.126416
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: EGTA significantly influenced StNOXB in T-2 toxin-treated slices. EGTA significantly influenced NOX activity on plant membrane and production of O2 − and H2 O2 . POD, CAT and SOD and ascorbate-glutathione cycle were markedly affected by Ca 2+ . Ca 2+ affects ROS metabolism and the activity of the antioxidant systems in tuber slices. Abstract: T-2 toxin at low concentrations can induce ROS accumulation and modulate host resistance in plants. NOX plays crucial roles in ROS production and is regulated by Ca 2+ via direct binding to EF-hand motifs. In this study, the effect of EGTA (Ca 2+ chelating agent) on the expression and enzymatic activity of NOX, as well as the activities and corresponding gene expressions involved in ROS metabolism and cell membrane integrity, were investigated in treated slices. Results indicated that EGTA treatment significantly affected gene expression and activity of NOX, and reduced ROS accumulation and cell membrane integrity and the enzymatic activities and gene expression involved in ROS metabolism when exposed to treatment. The addition of exogenous Ca 2+ restored the initial relative transcript abundance, ROS accumulation and their activities. Results suggest that Ca 2+ affected by EGTA plays a crucial role in NOX activity regulation, ultimately affecting ROS metabolism in slices induced by T-2 toxin.