Fabrication of Ligusticum chuanxiong polylactic acid microspheres: A promising way to enhance the hepatoprotective effect on bioactive ingredients. (1st July 2020)

• Record Type: Journal Article
• Authors: Ge, Huifang; Lin, Peixuan; Luo, Taiduan; Yan, Zhiming; Xiao, Jianbo; Miao, Song; Chen, Jicheng
• Is Part Of: Food chemistry. Volume 317(2020)
• Publication Date: 2020-07-01
• Subjects: Ligusticum chuanxiong -- Sustained-release -- Microspheres -- High fat diet -- Hepatoprotective effect; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2020.126377
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: Sustained-release microspheres encapsulation efficiency of TMP are more than 80% LCE-PLA microspheres duration of efficacy prolong nearly 5-fold. LCE-PLA microspheres cytotoxicity declined 25% than naked LCE. LCE-PLA microspheres had a significant hepatoprotective effect. Abstract: Ligusticum chuanxiong extract-polylactic acid sustained-release microspheres (LCE-PLA) are fabricated in this study for enhancing both duration and hepatoprotective efficacy of the main bioactive ingredients. LCE-PLA in vitro release, cytotoxicity and in vivo hepatoprotective effect were discussed to evaluate its efficiency and functionality. Results demonstrated that the optimal drug-loading rate and encapsulation efficiency of tetramethylpyrazine (TMP, the main active ingredient) were 8.19%, 83.72%, respectively. The LCE-PLA in vitro release of TMP showed prolong 5-fold and in vitro cytotoxicity declined 25.00% compared with naked LCE. After 6 weeks of in vivo intervention in high fat diet mice, both liver aspartate aminotransferase and alanine aminotransferase levels were higher in LCE-PLA group than LCE group. The above results indicated that TMP had a higher bioavailability of hepatoprotection when encapsulation of LCE-PLA was applied. The current study has provided a promising novel way to enhance the efficacy of short half-life ingredients.