Doxycycline transfer from substrate to white button mushroom (Agaricus bisporus) and assessment of the potential consumer exposure. (15th September 2020)

• Record Type: Journal Article
• Authors: Gbylik-Sikorska, Małgorzata; Gajda, Anna; Nowacka-Kozak, Ewelina; Posyniak, Andrzej
• Is Part Of: Food chemistry. Volume 324(2020)
• Publication Date: 2020-09-15
• Subjects: Doxycycline -- Button mushroom -- Transfer -- ADI; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2020.126867
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Experimental assessment of the potential doxycycline uptake by button mushrooms. UHPLC-MS/MS analysis for the determination of doxycycline in harvested mushrooms. The possibility of doxycycline transfer from contaminated substrate to mushroom. Estimation of hypothetical consumer exposure at below the ADI. Abstract: The presence of antibiotic residues in the food chain may pose a serious risk to human health. Locating and evaluating new sources of consumer exposure to antibiotic residues in food is a very important element of health protection. The possibility of doxycycline uptake from the substrate for mushroom cultivation by the white button mushroom ( Agaricus bisporus ) fruit body was investigated. Mushrooms were experimentally cultivated on substrate contaminated with 8 different doxycycline concentrations in substrate and analyte concentrations in mushroom samples were measured using ultra-high performance liquid chromatography – triple quadrupole tandem mass spectrometry (UHPLC–MS/MS) The obtained results clearly indicated that doxycycline transfers from contaminated substrate to mushrooms at concentrations ranging from 0.87 to 72.3 µg/kg, depending on substrate contamination concentration level and order of harvesting.