Applications of red pigments from psychrophilic Rhodonellum psychrophilum GL8 in health, food and antimicrobial finishes on textiles. (July 2020)

• Record Type: Journal Article
• Authors: Bisht, Garima; Srivastava, Srishti; Kulshreshtha, Ritu; Sourirajan, Anuradha; Baumler, David J; Dev, Kamal
• Is Part Of: Process biochemistry. Volume 94(2020)
• Page Start: 15
• Page End: 29
• Publication Date: 2020-07
• Subjects: Northwest Himalayas -- Leh Ladakh -- Pangong Tso Lake -- Rhodonellum psychrophilum -- Pigments -- Antimicrobial -- Antioxidant -- Cell proliferation -- textile dyeing -- Bio enhancer; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2020.03.021
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Red pigments of Rhodonellum psychrophilum GL8 exhibit antimicrobial and bio enhancer properties. Red pigments of R. psychrophilium GL8 possess antioxidant activity and thus are potentially useful as functional food ingredient. Red pigments of R. psychrophilium GL8 can be used in developing antimicrobial fabrics. Red pigments of R. psychrophilium GL8 stimulate cell proliferation of mouse cells in vitro . Abstract: A psychrophilic bacterium (named GL8) producing red pigments was isolated from the high altitude Pangong Tso Lake located in Leh Ladakh, India. Based on 16S rDNA sequencing amplicon of 1370 bp, the psychrophilic bacterium was identified as Rhodonellum psychrophilum ( R. psychrophilum GL8) and deposited in Gen Bank under accession no. MH031708.1.The red colored pigments from R. psychrophilum GL8 showed antimicrobial activity against E. coli, S. aureus, C. albicans, (MTCC 277, ATCC 90028) and S. cerevisiae (H1086) with minimum inhibitory concentrations from 31.25 μg/mL to 500 μg/mL. Red colored pigments also showed synergistic antifungal activity when combined with fluconazole and amphotericin B against C. albicans (MTCC 277 and ATCC 90028) and S. cerevisiae ; vancomycin and erythromycin against E. coli and S. aureus . The red pigments showed antioxidant activity with an IC50 of 13.159 μg/mL, LC–MS/MS analysis demonstrated that red pigment extracts contained a mixture of 2-methyl-3-butyl-prodigine, Prodigiosin, 2-methyl-3hexyl-prodigine, 3, 4-Didehydrorhodopsin, anhydrorhodovibrin, alloxanthin and Tetradecanoyl-hexadecanoyl compounds. Red pigment extracts were used to develop antimicrobial fabrics and did not show any cytotoxicity to U87MG human glioblastoma cell line, but showed a marginal growth inhibition to A172 human glioblastoma cell lines. In contrast, the red pigment extracts showed significant growth stimulation on L929 mouse fibroblast cell lines.