Low-molecular weight peptides isolated from seahorse (Hippocampus abdominalis) improve vasodilation via inhibition of angiotensin-converting enzyme in vivo and in vitro. (August 2020)

• Record Type: Journal Article
• Authors: Je, Jun-Geon; Kim, Hyun-Soo; Lee, Hyo-Geun; Oh, Jae-Young; Lu, Yu An; Wang, Lei; Rho, Sum; Jeon, You-Jin
• Is Part Of: Process biochemistry. Volume 95(2020)
• Page Start: 30
• Page End: 35
• Publication Date: 2020-08
• Subjects: ACE angiotensin-converting enzyme -- SHP seahorse hydrolyzed by protamex -- SHP-I over 10 kDa fraction derived from seahorse hydrolyzed by protamex -- SHP-II 5–10 kDa fraction derived from seahorse hydrolyzed by protamex -- SHP-III below 5 kDa fraction derived from seahorse hydrolyzed by protamex -- Q-TOF quadrupole-time-of-flight -- SHR spontaneously hypertensive rat -- UF ultrafiltration -- RP-HPLC reverse-phase high performance liquid chromatography -- HUVECs human umbilical vein endothelial cells -- p-eNOS phosphorylation of endothelial nitric-oxide synthase -- NO nitric oxide -- IC50 the half maximal inhibitory concentration; Seahorse -- Hippocampus abdominalis -- Angiotensin-converting enzyme -- Peptide -- Vasodilation; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2020.04.016
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Seahorse has long been used for the purpose of improving male sexual function; related to blood pressure and blood vessels. Seahorse was hydrolyzed by Protamex (SHP) and later further separated by the ultrafiltration and sephadex G-10. SHP inhibits angiotensin-converting enzyme and increase nitric oxide production, showing an anti-hypertension effect. Abstract: According to relevant literature, the consumption of seahorses has been used to increase male functions by improving blood flow and decreasing blood pressure. Thus, it was theorized that seahorses may also be effective against hypertension. Herein, vasodilation caused via the inhibitory effect of angiotensin-converting enzymes (ACEs) in peptides was elucidated by studying the seahorses ( Hippocampus abdominalis ) farmed at Jeju in South Korea. Hydrolysate was prepared using the Protamex (SHP) enzyme. The ultrafiltration system was adopted to separate certain fractions from SHP according to different molecular weights (SHP-I, MW > 10 kDa; SHP-II, MW = 5−10 kDa; SHP-III, MW < 5 kDa). The fraction with the lowest molecular weight (SHP-III) and with a low IC50 value (0.044 ± 0.005) for the ACE inhibitory effect was further separated by Sephadex G-10. Three ACE inhibitory peptides from SHP-III were isolated and identified using the Q-TOF mass spectrometer (Ala-Pro-Thr-Leu, Cys-Asn-Val-Pro-Leu-Ser-Pro, and Pro-Trp-Thr-Pro-Leu). Furthermore, SHP-III-induced systolic blood pressure varied with the concentrations, as observed in the spontaneously hypertensive rat (SHR). SHP and the isolated peptides were observed to show vasodilation via ACE inhibitions and resulted in lowering the blood pressure of the SHR. These results imply that peptides from seahorses can augment male functions.