Enzyme-assisted extraction of a cup plant (Silphium perfoliatum L.) Polysaccharide and its antioxidant and hypoglycemic activities. (May 2020)

• Record Type: Journal Article
• Authors: Guo, Yang; Shang, Hongmei; Zhao, Jiangchao; Zhang, Hexiang; Chen, Shilun
• Is Part Of: Process biochemistry. Volume 92(2020)
• Page Start: 17
• Page End: 28
• Publication Date: 2020-05
• Subjects: Cup plant (Silphium perfoliatum L.) -- Polysaccharides -- Optimization -- Activity; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2020.03.005
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Polysaccharides from cup plant ( Silphium perfoliatum L.) were named CPP. The enzyme-assisted extraction conditions of CPP were optimized by RSM. A purified polysaccharide component (CPP-1.2) was obtained from CPP. CPP-1.2 showed scavenging abilities on DPPH and ABTS radicals. CPP-1.2 had notable inhibition activities on α-amylase and α-glucosidase. Abstract: The optimum enzyme-assisted extraction conditions of cup plant ( Silphium perfoliatum L.) polysaccharides (CPP) were obtained by response surface methodology (RSM), and were found to be an enzymatic hydrolysis time of 66 min, a liquid-solid ratio of 22 mL/g, pH 5.1, and an enzyme-complex dosage of 1.59 %, allowing a CPP yield of 13.69 %. DEAE-52 cellulose and Sepharose CL-6B columns were used to purify the CPP. A purified polysaccharide component, CPP-1.2, was obtained and further analyzed for its characteristics, antioxidant properties, and hypoglycemic activity. The monosaccharide composition of CPP-1.2 was mannose, galacturonic acid, glucose, galactose, and arabinose with a molar percentage ratio of 2.24:16.34:44.17:24.28:12.97. The 1, 1-Diphenyl-2-picrylhydrazyl (DPPH) and 2, 2-Azino- bis -(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) radical scavenging abilities of CPP-1.2 (1.0 mg/mL) reached 95.20 % and 93.69 % that of ascorbic acid (1.0 mg/mL), respectively. Moreover, CPP-1.2 presented significantly higher inhibitory properties on the activities of α-amylase and α-glucosidase than acarbose at each concentration tested. These results suggest that CPP-1.2 possesses the potential to be used as an antioxidant and hypoglycemic agent.