Biochemical and kinetic evaluation of lipase and biosurfactant assisted ex novo synthesis of microbial oil for biodiesel production by Yarrowia lipolytica utilizing chicken tallow. (August 2020)

• Record Type: Journal Article
• Authors: Radha, P.; Prabhu, Keerthana; Jayakumar, Anjali; AbilashKarthik, S.; Ramani, K.
• Is Part Of: Process biochemistry. Volume 95(2020)
• Page Start: 17
• Page End: 29
• Publication Date: 2020-08
• Subjects: Microbial oil -- Yarrowia lipolytica -- Biodiesel -- Chicken tallow -- Lipase -- Biosurfactant; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2020.05.009
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Chicken tallow was used as the substrate to produce microbial oil. Biomass and lipid yields were enhanced through various optimization studies. The lipid accumulation process was examined through various instrumental analyzes. Co synthesis of lipase and biosurfactant besides lipid accumulation was observed. Microbial oil was transesterified to biodiesel, and its properties were predicted. Abstract: The present study explores the production of biodiesel, a sustainable replacement for depleting fossil fuel by utilizing microbial oil, which was procured from Yarrowia lipolytica employing chicken tallow as the carbon substrate. Chicken tallow, yeast extract, and MgSO4 ·7H2 O were screened for biomass production through Plackett–Burman design. Further, Box–Behnken design analysis was performed, and the optimal concentration of the medium variables was found to be 20 g/L of chicken tallow, 7.0 g/L of yeast extract, and 0.45 g/L of MgSO4 ·7H2 O.The various parameters viz., pH (6), temperature (30 °C), RPM (150), inoculum volume (5%, v/v), and C/N ratio (100) were optimized for maximal biomass and lipid yield, and lipid content. Nile red-stained cells were observed for intracellular lipid bodies using fluorescence microscopy, and its fluorescence intensity was measured bythe flow cytometer. The dimorphic transition and substrate assimilation of Y. lipolytica were analyzed using scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy (FT-IR). Batch kinetic studies revealed the concomitant synthesis of microbial lipid (4.16 g/L), lipase (43 U/mL), and biosurfactant (1.41 g/L). The GC-MS analysis of microbial oil presented the fatty acid profile as oleic acid (49.15%), palmitic acid (29.83%), stearic acid (11.43%), linoleic acid (3.83%), palmitoleic acid (3.77%), and myristic acid (1.32%).