Dietary Luffa cylindrica (L.) Roem promotes branched-chain amino acid catabolism in the circulation system via gut microbiota in diet-induced obese mice. (1st August 2020)

• Record Type: Journal Article
• Authors: Zhang, Lu; Yue, Yunshuang; Shi, Mengxuan; Tian, Meiling; Ji, Junfu; Liao, Xiaojun; Hu, Xiaosong; Chen, Fang
• Is Part Of: Food chemistry. Volume 320(2020)
• Publication Date: 2020-08-01
• Subjects: ALT alanine aminotransferase -- AN ampicillin and neomycin -- AST aspartate aminotransferase -- AUC the area under the curve -- BCAA branched-chain amino acid -- BCATm mitochondrial branched-chain aminotransferase -- BCKA branched-chain ketoisocaproic acid -- BCKDK branched-chain alpha-keto acid dehydrogenase kinase -- BMI body mass index -- DIO die-induced obese -- FSG fasting glucose -- FSI fasting insulin -- HE hematoxylin and eosin -- HOMA-IR homeostasis model assessment of insulin resistance -- HFD high-fat diet -- IGTT intraperitoneal glucose tolerance test -- Ile isoleucine -- IR insulin resistance -- ITT intraperitoneal insulin test -- KEGG Kyoto Encyclopedia of Genes and Genomes -- Leu leucine -- LEfSe linear discriminant analysis effect size -- Luffa cylindrica (L.)Roem Luffa -- MRM multiple reaction monitoring -- NCD normal control diet -- NMR nuclear magnetic resonance -- OTU operational taxonomic unit -- PAS periodic acid schiff -- PCoA principal coordinate analysis -- PERMANOVA permutational multivariate analysis of variance -- PICRUSt phylogenetic investigation of communities by reconstruction of unobserved states -- RDA redundancy -- SCFA short-chain fatty acid -- SPF specific-pathogen free -- TC total cholesterol -- TG total triglycerides -- Val valine; Obesity -- Insulin resistance -- Luffa cylindrica -- BCAA catabolism -- Gut microbiota -- Antibiotic-treated mice; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2020.126648
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: BCAAs can be regarded as serum biomarkers for obesity. Luffa potentially suppressed bacterial BCAA biosynthesis. Luffa -affected bacterial metabolites triggered tissue-specific BCAA catabolism. Luffa improved circulating BCAA dysfunction via gut microbiota to attenuate obesity. Abstract: High circulating branched-chain amino acid (BCAA) levels can be diagnosis indicators for obesity. Luffa cylindrica ( luffa ) is one of vegetables against obesity. However, whether the anti-obesity of luffa is associated with BCAA metabolism and gut microbiota remains unknown. Here, we used conventionally raised diet-induced obese (DIO) mice to prove dietary luffa could reduce higher circulating BCAA levels and upregulate the tissue-specific expressions of BCAA-catabolizing enzymes. Meanwhile, dietary luffa selectively decreased the relative abundances of g_Enterortabdus, g_Eubacterium_xylanophilum_group and g_Butyricicoccus that exhibited significantly positive correlations with BCAA levels, BMI and HOMA-IR. Bacterial functionality prediction indicated dietary luffa potentially inhibited bacterial BCAA biosynthesis for reducing BCAAs supplementation. More importantly, dietary luffa had no impacts on BCAA catabolism in germ-free-mimic DIO mice. Thus, dietary luffa improved BCAA dysfunction via gut microbiota to attenuate obesity. This study offers a novel insight into dietary intervention against obesity from the aspect of gut microbiota-amino acid metabolism.