Action of endogenous proteases on texture deterioration of the bay scallop (Argopecten irradians) adductor muscle during cold storage and its mechanism. (1st September 2020)

• Record Type: Journal Article
• Authors: Liu, Bing; Liu, Zi-qiang; Li, De-yang; Yu, Man-man; Liu, Yu-xin; Qin, Lei; Zhou, Da-yong; Shahidi, Fereidoon; Zhu, Bei-wei
• Is Part Of: Food chemistry. Volume 323(2020)
• Publication Date: 2020-09-01
• Subjects: Scallop -- Endogenous proteases -- Textural properties -- Myofibrillar proteins -- Connective tissue proteins -- Multivariate statistical techniques; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2020.126790
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Endogenous proteases cause texture deterioration of scallop during cold storage. Endogenous cysteine proteases mainly degrade myofibrillar proteins. Endogenous serine proteases degrade myofibrillar and connective tissue proteins. Endogenous proteases cause the changes of microstructure and the reduction of WHC. Serine- and cysteine-protease inhibitors prevent the texture deterioration. Abstract: Texture deterioration occurs in adductor muscle of scallop ( Argopecten irradians ) (AMS) after 5 d of cold storage. Principal component analysis indicated the texture deterioration resulted in significant decrease of hardness, springiness, adhesiveness and chewiness, but significantly increased cohesiveness. Endogenous proteases degraded structural proteins, among which cysteine proteases were mainly responsible for myofibrillar proteins (MPs) degradation, while serine proteases degraded both MPs and connective tissue proteins. Pearson coefficient analysis showed that texture indicators significantly correlated with structural protein indicators in AMS. To be more specific, the hardness, springiness, adhesiveness and chewiness negatively correlated with myofibrillar fragmentation index, soluble hydroxyproline (Hyp) and soluble glycosaminoglycans, but positively correlated with solubility of MPs and water holding capacity. Meanwhile, the cohesiveness positively correlated with soluble Hyp. The Taylor diagram and Hierarchical cluster analysis confirmed that the inhibitors of cysteine and serine proteases could effectively retard textural deterioration of AMS during 5 d of cold storage.