Policosanol profiles and adenosine 5′-monophosphate-activated protein kinase (AMPK) activation potential of Korean wheat seedling extracts according to cultivar and growth time. (1st July 2020)

• Record Type: Journal Article
• Authors: Ra, Ji-Eun; Woo, So-Yeun; Lee, Kwang-Sik; Lee, Mi Ja; Kim, Hyun Young; Ham, Hyeon Mi; Chung, Ill-Min; Kim, Du Hyun; Lee, Jin Hwan; Seo, Woo Duck
• Is Part Of: Food chemistry. Volume 317(2020)
• Publication Date: 2020-07-01
• Subjects: Wheat seedling -- Policosanol -- Octacosanol -- AMPK -- Growth times -- GC–MS; Eicosanol (PubChem CID 12404) -- Heneicosanol (PubChem CID 85014) -- Docosanol (PubChem CID12620) -- Tricosanol (PubChem CID 18431) -- Tetracosanol (PubChem CID 10472) -- Hexacosanol (PubChem CID 68171) -- Heptacosanol (PubChem CID 74822) -- Octacosanol (PubChem CID 68406) -- Triacontanol (PubChem CID 68972); AMPK adenosine 5′-monophosphate-activated protein kinase -- MSTFA N-methyl-N-(trimethylysilyl)trifluoroacetamide -- MTT Thiazolyl blue tetrazolium bromide -- GC–MS Gas chromatography-mass spectrometry -- HepG2 Liver hepatoceullular carcinoma; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2020.126388
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Policosanols of wheat seedlings showed remarkable differences in cultivars and growth times. The highest average policosanol contents showed 738.7 mg/100 g on 6 days after sowing. Octacosanol was the predominant component in various cultivars of wheat seedlings. AMPK activation exhibited considerable differences in growth times of wheat seedlings. Octacosanol in wheat seedlings possessed potent AMPK activator. Abstract: Policosanols is a health promoting aliphatic alcohol known as lipid-lowing agent. To enable maximising the functional properties of wheat, this research investigates the policosanol profiles and adenosine 5′-monophosphate-activated protein kinase (AMPK) activation potential of Korean wheat seedlings according to cultivars and growth times. GC-MS revealed six policosanols that differed markedly in content between 17 cultivars, especially, octacosanol (8 ) showed the most predominant component (49–83%), varying significantly in average concentrations with growth times as 361.4 (3 days) → 613.0 (6 days) → 203.1 (9 days) → 196.5 (12 days) → 50.9 mg/100 g (19 days). The highest average policosanol (738.7 mg/100 g) exhibited after 6 days, while the lowest was 104.4 mg/100 g on 19 days. Moreover, the wheat cultivars including Shinmichal 1, Anbaek, Namhae, and Joah at 6 days may be recommended as potential sources because of high policosanols (921.7–990.6 mg/100 g). Western blot analysis revealed markedly higher AMPK activation in cells treated with the hexane extracts (150–370% at 100 μg/ml) and octacosanol (8 ) possessed potent AMPK activator (control; 100 → 280% at 200 μg/ml). It is confirmed that the AMPK activation by wheat seedlings are positively related to the highest policosanol content at the 6 days of growth time, independent of the cultivar. Our results may be contributed to enhance the wheat value regarding development of new cultivars and functional foods.