Dietary galactosyl and mannosyl carbohydrates: In-vitro assessment of prebiotic effects. (1st November 2020)

• Record Type: Journal Article
• Authors: Wei, Xinyi; Fu, Xiaodan; Xiao, Mengshi; Liu, Zhemin; Zhang, Lanwei; Mou, Haijin
• Is Part Of: Food chemistry. Volume 329(2020)
• Publication Date: 2020-11-01
• Subjects: Hydrolyzed guar gum -- Manno-oligosaccharide -- Galacto-oligosaccharide -- Fecal microbiota -- Short-chain fatty acid; ANOVA analysis of variance -- BB Bifidobacterium breve DNKD1 -- BBI B. bifidum DNKD2 -- BI B. infantis ATCC 15697 -- BL B. lactis KTV9 -- CB Clostridium butyricum CDC 51208 -- EC Escherichia coli ATCC 25922 -- GMOS hydrolyzed guar gum, Mw <1 kDa -- GMPS hydrolyzed guar gum, Mw 1–10 kDa -- GOS galacto-oligosaccharide -- HPLC high-performance liquid chromatography -- MOS manno-oligosaccharide -- LS Lactobacillus sakei CGMCC 1.6 -- Mw molecular weight -- MWCO molecular weight cut-off -- NDC non-digestible carbohydrate -- OTUs operational taxonomic units -- PP Pediococcus pentosaceus CY-27f -- SCFA short-chain fatty acid -- SD standard deviation -- TLC thin-layer chromatography -- Tukey's HSD Tukey's honestly significant difference; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2020.127179
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Hydrolyzed guar gum, MOS, and GOS showed selective prebiotic effects. GOS and GMOS promoted the growth of bifidobacteria and C. butyricum, respectively. GMOS gave higher yield of lactate, propionate, and butyrate than other carbohydrates during human fecal fermentation. Prebiotic activity was related to the glycoside compositions and molecular weights. Abstract: The prebiotic activities of hydrolyzed guar gum (GMOS, <1 kDa; GMPS, 1–10 kDa), manno-oligosaccharide (MOS, <1 kDa), and galacto-oligosaccharide (GOS, <1 kDa) were evaluated by in vitro fermentation. The tested carbohydrates showed selective prebiotic effects on bacterial growth, short-chain fatty acid (SCFA)-production, and substrate consumption. GOS and GMOS markedly promoted the growth of bifidobacteria and Clostridium butyricum, respectively, whereas MOS showed the strongest butyrogenic effect. Moreover, SCFA production in the hydrolyzed guar gum groups was closely related to the varied molecular weight (Mw) of the hydrolysate. During in vitro fermentation with human fecal inocula, GMOS gave the highest yields of lactate, propionate, and butyrate after 48 h fermentation. Combined application of MOS and C. butyricum increased the abundance of Clostridiaceae_1. Overall, our results indicate that galactosyl and mannosyl carbohydrates have individualized prebiotic effects which are associated with their chemical structures including their glycoside composition and Mw.