Involvement of protein S-nitrosylation in regulating beef apoptosis during postmortem aging. (1st October 2020)

• Record Type: Journal Article
• Authors: Hou, Qin; Liu, Rui; Tian, Xiaona; Zhang, Wangang
• Is Part Of: Food chemistry. Volume 326(2020)
• Publication Date: 2020-10-01
• Subjects: Protein S-nitrosylation -- Apoptosis -- Morphological analysis -- Caspase -- Postmortem aging; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2020.126975
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Beef protein S-nitrosylation level was promoted by GSNO and restrained by l -NAME. Apoptosis-related morphological changes were retarded by protein S-nitrosylation. The apoptotic nuclei counts were decreased by GSNO and increased by l -NAME. Protein S-nitrosylation negatively regulated caspase activity and activation. Abstract: This study was the first attempt to explore the effect of protein S-nitrosylation on the progress of apoptosis in postmortem beef semimembranosus muscle (SM). Five beef SM were incubated with S-nitrosoglutathione (GSNO, nitric oxide donor), control, or Nω-nitro-l -arginine methyl ester hydrochloride (l -NAME, nitric oxide synthase inhibitor). Results suggest that compared to the control, more chromatin condensation, nucleus fragmentation, apoptotic body formation, and mitochondrial swelling were observed in the l -NAME group while these apoptosis-related morphological changes were retarded in the GSNO group. Notably, there were fewer apoptotic nuclei in the GSNO group and more apoptotic nuclei in the l -NAME group compared to the control ( P < 0.05). Additionally, caspase-3 and -9 activities and caspase-3 activation were greatly decreased by GSNO treatment and increased by l -NAME treatment ( P < 0.05). The morphological and biochemical results indicate that protein S-nitrosylation could play a negative regulatory role in beef apoptosis during postmortem aging.