The novel peptides ICRD and LCGEC screened from tuna roe show antioxidative activity via Keap1/Nrf2-ARE pathway regulation and gut microbiota modulation. (15th October 2020)

• Record Type: Journal Article
• Authors: Han, Jiaojiao; Huang, Zhongbai; Tang, Shasha; Lu, Chenyang; Wan, Haitao; Zhou, Jun; Li, Ye; Ming, Tinghong; Jim Wang, Zaijie; Su, Xiurong
• Is Part Of: Food chemistry. Volume 327(2020)
• Publication Date: 2020-10-15
• Subjects: DPPH 1, 1-diphenyl-2-picrylhydrazyl -- IPA 3-indolepropionic acid -- SCFA Short-chain fatty acid -- ROS Reactive oxygen species -- GSH-Px Glutathione peroxidase -- SOD Superoxide dismutase -- BHT 2, 6-ditert-butyl-4-methyl phenol -- BHA Butyl hydroxy anisole -- MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolinum bromide] -- LEfSe Discriminant analysis effect size -- RDA Redundancy analysis -- OTU Operational taxonomic unit -- PCoA Principal coordinate analysis -- qRT-PCR Quantitative real time- polymerase chain reaction -- TNF-α Tumor necrosis factor-α -- IL-6 Interleukin 6; Peptide -- High throughput strategty -- Tuna roe -- Antioxidant -- Gut microbiota -- Metabolism; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2020.127094
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Two antioxidant peptides ICRD and LCGEC were screened and identified from tuna roe. ICRD and LCGEC regulated Keap1/Nrf2-ARE pathway transcription in cell and mice. LCGEC regulated gut microbiota structure and metabolism in mice. Abstract: In this study, a high-throughput strategy combined with MALDI TOF/TOF-MS and Discovery Studio 2017 was developed to screen peptides with certain functions from hydrolysate. Two dominant peptides, Ile-Cys-Arg-Asp (ICRD) and Leu-Cys-Gly-Glu-Cys (LCGEC), were predicted to have antioxidant activity by Discovery Studio 2017. Then the activity in vitro of peptides had been confirmed via DPPH assay. Both two peptides decreased apoptosis induced by UVB treatment in HaCaT cells and altered Keap1/Nrf2-ARE pathway transcription. Furthermore, the antioxidant activity of LCGEC was achieved after 6-week treatment in mice via regulating the Keap1/Nrf2-ARE pathway, inhibiting the release of proinflammatory cytokines, increasing the abundance of 3-indolepropionic acid and short-chain fatty acids production in feces and modulating gut microbiota composition. This study provided two tuna roe peptides with in vitro and in vivo antioxidant activity.