A rapid and convenient method for on-site detection of MON863 maize through real-time fluorescence recombinase polymerase amplification. (15th September 2020)

• Record Type: Journal Article
• Authors: Wang, Xiaofu; Xie, Shilong; Chen, Xiaoyun; Peng, Cheng; Xu, Xiaoli; Wei, Wei; Ma, Tongfu; Cai, Jian; Xu, Junfeng
• Is Part Of: Food chemistry. Volume 324(2020)
• Publication Date: 2020-09-15
• Subjects: Genetically modified crop -- Recombinase polymerase amplification (RPA) -- Real-time fluorescence -- On-site detection -- MON863; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2020.126821
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: A method for real-time and on-site detection of MON863 was proposed. The RT-RPA assay exhibited high specificity and sensitivity (LOD of 20 copies). The RT-RPA assay could detect MON863 within 10 min, which is quicker than RT-PCR. The practicability of this method for on-site detection is verified. Abstract: As large-scale planting of genetically modified (GM) crops increases, the development of a rapid and convenient method for on-site detection of GM crops is important. We combined the advantages of recombinase polymerase amplification (RPA) and fluorescence detection to establish a rapid, sensitive, specific, and simple detection platform for on-site detection of MON863 maize. Test samples were added directly to the platform after simple pre-treatment with a DNA extraction-free method. Results were obtained through real-time monitoring with a portable instrument, which facilitated sample-in/answer-out on-site detection. The entire detection process, including sample preparation, RPA and identification of amplification results, was accomplished in approximately 10 min. Furthermore, the detection was achieved with a simple and inexpensive portable device. This method has high potential for application in other fields requiring rapid detection of DNA targets, such as in field research, resource-limited areas, and science education.