Dynamic variations of microbial community structure in Myriophyllum aquaticum constructed wetlands in response to different NH4+-N concentrations. (June 2020)

• Record Type: Journal Article
• Authors: Xu, Lina; Zhang, Bao; Peng, Xiawei; Zhang, Xupo; Sun, Bo; Sun, Haishu; Jiang, Cancan; Zhou, Sining; Zeng, Xiangui; Bai, Zhihui; Xu, Shengjun; Zhuang, Xuliang
• Is Part Of: Process biochemistry. Volume 93(2020)
• Page Start: 55
• Page End: 62
• Publication Date: 2020-06
• Subjects: Myriophyllum aquaticum -- Constructed wetlands -- Ammonium nitrogen concentrations -- Microbial community structure -- Denitrifiers; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2020.02.028
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: CW under 105 mg/L NH4 + -N concs achieved optimal N removal efficiency. Higher abundance of NarG and NosZ genes were found in high NH4 + -N concs. CWs. Denitrifiers including Rhodococcus.sp were enriched under high NH4 + -N concs. CWs. Increasing NH4 + -N concs. led to enhanced interactions among microbial community. Abstract: Constructed wetlands (CWs) have received increasing attentions for their N removal performances, especially regarding NH4 + -N. Different influent NH4 + -N concentration may influence N removal efficiency in practice, while the effects of different NH4 + -N concentrations on microorganisms removing N in CWs are poorly understood. In this study, surface flow CWs planted with Myriophyllum (M) . aquaticum were established to investigate the influences of different NH4 + -N concentrations on the composition, structure, and interactions of microbial community. Our findings suggested 105 mg/L NH4 + -N CWs achieved highest N removal rate, removing 89.30 % NH4 + -N and 92.34 % TN from the influent. The results of real-time quantitative polymerase chain reactions (qPCR) indicated abundances of nitrifying genes ( nxrA ) and denitrifying genes ( narG, nirS, nirK, and nosZ ) were increased by increasing NH4 + -N concentrations, and the strongest effects were observed in narG (8-fold) and nosZ genes (11-fold). Different NH4 + -N concentrations was proved to alter composition and structure of microbial communities via high-throughput sequencing, e.g. denitrifiers including Brevendomonas.sp, Dokdonella.sp and Rhodococcus.sp were enriched obviously with increasing NH4 + -N concentrations. In addition, network showed interactions among microbial populations and positive interactions were dramatically shifted and enhanced by increasing NH4 + -N concentrations.