A new analytical method for the simultaneous quantification of isoflavones and lignans in 25 green coffee samples by HPLC-MS/MS. (30th September 2020)

• Record Type: Journal Article
• Authors: Angeloni, Simone; Navarini, Luciano; Khamitova, Gulzhan; Maggi, Filippo; Sagratini, Gianni; Vittori, Sauro; Caprioli, Giovanni
• Is Part Of: Food chemistry. Volume 325(2020)
• Publication Date: 2020-09-30
• Subjects: DAD diode-array detector -- ESI electrospray ionization -- GC–MS gas chromatography-mass spectrometry -- HPLC-MS/MS high performance liquid chromatography-tandem mass spectrometry -- ID isotope dilution -- LARI lariciresinol -- LOD limit of detection -- LOQ limit of quantification -- MAT matairesinol -- MRM multiple reaction monitoring -- n.d. not detectable -- RSD relative standard deviation -- SECO secoisolariciresinol -- SNR signal-to-noise ratio -- SPE solid-phase extraction -- TIC total ion current chromatogram; Isoflavone -- Lignan -- Green coffee -- HPLC-MS/MS -- Phytoestrogen -- Extraction process; Biochanin A (PubChem CID 5280373) -- Daidzein (PubChem CID 5281708) -- Daidzin (PubChem CID 107971) -- Formononetin (PubChem CID 5280378) -- Genistein (PubChem CID 5280961) -- Genistin (PubChem CID 5281377) -- Lariciresinol (PubChem CID 332427) -- Matairesinol (PubChem CID 119205) -- Secoisolariciresinol (PubChem CID 65373); Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2020.126924
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: A new analytical method for lignan and isoflavone quantitation has been developed. Base hydrolysis followed by enzymatic digestion was the best extraction process. Lignans occurred in higher concentrations than isoflavones. Matairesinol was not present in any samples. Ethiopian green coffees may be a good source of lignans. Abstract: Green coffee, the raw material of roasted coffee and coffee beverages, is one of the most widely traded commodities worldwide. There is a dearth of studies on its content in phytoestrogens such as isoflavones and lignans. Previously, we developed an efficient method for the simultaneous quantification of 6 isoflavones (daidzin, genistin, daidzein, genistein, formononetin and biochanin A) and 3 lignans (secoisolariciresinol, matairesinol and lariciresinol) in green coffee by using HPLC-MS/MS. Several extraction processes were evaluated and the best performing, base hydrolysis followed by enzymatic digestion, was validated and used to analyse 25 different coffee samples, 1 Coffea canephora and 24 Coffea arabica, from different countries. Lignans (total content: 286.5–8131.8 µg kg −1 ) were found in higher concentration than isoflavones (total content: 3.4–300.0 µg kg −1 ) and the most abundant were secoisolariciresinol (172.6–5714.1 µg kg −1 ) and lariciresinol (113.9–2417.7 µg kg −1 ). Notably, the Ethiopian coffee samples contained the highest levels of these compounds.