Bioconversion of a ganoderic acid 3-hydroxy-lanosta-8, 24-dien-26-oic acid by a crude enzyme from Ganoderma lucidum. (August 2020)

• Record Type: Journal Article
• Authors: Cai, Siqin; Xiao, Han; Wang, Xiaozheng; Lin, Shuangjun; Zhong, Jian-Jiang
• Is Part Of: Process biochemistry. Volume 95(2020)
• Page Start: 12
• Page End: 16
• Publication Date: 2020-08
• Subjects: Basidiomycete mushroom -- Ganoderma lucidum -- triterpenoid -- ganoderic acid -- bioconversion; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2020.05.002
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Post-modification of 3-hydroxy-lanosta-8, 24-dien-26 oic acid (HLDOA) was found. The conversion product of HLDOA was 3-oxo-lanosta-8, 24-dien-26 oic acid (OLDOA). The work is useful to biomanufacture and pathway elucidation of GAs. Abstract: Ganoderic acids (GAs) are oxygenated lanostane-type triterpenoids from the traditional medicinal mushroom Ganoderma lucidum and of significant biological activities. Although a ganoderic acid 3-hydroxy-lanosta-8, 24-dien-26-oic acid (HLDOA) was found to be biosynthesized from lanosterol, further post-modification of HLDOA is yet unclear. In this work, by using HLDOA as the substrate and a crude enzyme from G. lucidum as the biocatalyst, we observed a new peak in liquid chromatography from the reaction system. The product was purified and identified to be 3-oxo-lanosta-8, 24-dien-26-oic acid (OLDOA), which may be converted from HLDOA by a putative dehydrogenase of G. lucidum . The work is useful to future manufacture of GAs as well as their biosynthetic pathway elucidation.