Fabrication of an organic–inorganic nanocomposite carrier for enzyme immobilization based on metal–organic coordination. (August 2020)

• Record Type: Journal Article
• Authors: Qiu, Xiang; Xiang, Xinran; Liu, Tingting; Huang, He; Hu, Yi
• Is Part Of: Process biochemistry. Volume 95(2020)
• Page Start: 47
• Page End: 54
• Publication Date: 2020-08
• Subjects: Nanocomposite -- Mesoporous material -- Chitosan -- Ionic liquid -- Enzyme immobilization; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2020.05.007
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: PPL was immobilized on organic-inorganic nanocomposites. Immobilized PPL showed high immobilization yield and activity recovery. Immobilized PPL exhibited superior environmental tolerance and stability. Immobilized PPL presented improved reusability. Abstract: An organic–inorganic nanocomposite which combined mesoporous silica SBA-15 and chitosan using a carboxyl functionalized ionic liquid as the bridging agent (SBA@CS) was successfully fabricated, and was used to immobilize porcine pancreas lipase (PPL) by physical adsorption, cross-linking and metal–organic coordination, respectively. The as-prepared carriers were characterized by scanning electron microscopy, Fourier transform infrared and energy-dispersive X-ray spectroscopy. Compared with immobilization onto the pure mesoporous silicon material SBA-15, all the batches of PPL immobilized onto organic–inorganic nanocomposites showed higher activity, improved stability and reusability as well as better resistance to pH and temperature changes. Among the immobilized PPLs, immobilization based on Co 2+ coordination (SBA@CS-Co-PPL) produced the best enzymatic properties. The maximum immobilization efficiency and specific activity of 79.6% and 1975.8 U g −1 were obtained with SBA@CS-Co, separately. More importantly, the activity of immobilized enzyme can still maintain 84.0% after 10 times of reuse. These results demonstrated that thus prepared organic–inorganic nanocomposite could be an ideal carrier for enzyme immobilization by metal–organic coordination.