Rational design and synthesis of molecularly imprinted polymers (MIP) for purifying tylosin by seeded precipitation polymerization. (July 2020)

• Record Type: Journal Article
• Authors: Zeng, Hainan; Yu, Xue; Wan, Junfen; Cao, Xuejun
• Is Part Of: Process biochemistry. Volume 94(2020)
• Page Start: 329
• Page End: 339
• Publication Date: 2020-07
• Subjects: Molecularly imprinted polymer -- Core-shell structure -- Molecular simulation -- Solid phase extraction -- Tylosin -- Precipitation polymerization; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2020.03.025
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: The MIP prepared has high adsorption capacity and good selectivity. Molecular simulation considering solvent was used to select proper monomer. Seeded precipitation polymerization was proposed to obtain micron-sized MIP. Two steps adsorption model of MIP was demonstrated by Scatchard method. Purifying tylosin from fermentation broth was achieved by solid phase extraction. Abstract: A useful approach was proposed to easily synthesize molecularly imprinted adsorbent for the purification of tylosin from broth. Firstly, by molecular simulation based on density functional theory, methacrylic acid was chosen as a functional monomer by comparing the binding energy. Second, a novel method of polymerization based on precipitation polymerization with added seeds was used in water-mixed solvent for the preparation of water-compatible micron-sized MIP. Its static adsorption capacity for tylosin in aqueous solution was estimated to be 106.5 mg/g with the highest imprinting factor (IF) of 3.6. The selectivity coefficient (α) of tylosin to desmycosin was 3.3. The antibiotic in fermentation broth could be purified by means of molecularly imprinted solid phase extraction (MISPE), which allows MIP to be used for the purification of tylosin from a complex sample.