A new member of the surfactin family produced by Bacillus subtilis with low toxicity on erythrocyte. (July 2020)

• Record Type: Journal Article
• Authors: Fei, Dan; Liu, Fang-Fang; Gang, Hong-Ze; Liu, Jin-Feng; Yang, Shi-Zhong; Ye, Ru-Qiang; Mu, Bo-Zhong
• Is Part Of: Process biochemistry. Volume 94(2020)
• Page Start: 164
• Page End: 171
• Publication Date: 2020-07
• Subjects: Bacillus subtilis -- Microbial lipopeptide -- Surfactin -- Surface activity -- Hemolysis; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2020.04.022
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Bacillus subtilis TD 7 produced a new member with the shortest hydrophobic chain among the surfactin family. Its fatty acid part contained both of the iso -C11 and anteiso -C11 β -hydroxy fatty acids. Its peptide sequence was N -Glu-Leu-Leu-Val-Asp-Leu-Leu- C . Surfactin-C11 exhibited good surface active. Surfactin-C11 showed low toxicity on erythrocyte. Abstract: A new member of the surfactin family of microbial lipopeptides has been isolated from the cell broth of Bacillus subtilis TD 7 by reverse phase high performance liquid chromatography (RP-HPLC), and identified by FT-IR, GC–MS, LC–MS/MS, 1 H-NMR and 13 C-NMR. The surfactin-C11 consists of a heptapeptide with the sequence of N - Glu - Leu - Leu - Val - Asp - Leu - Leu - C, and a β -hydroxy fatty acid chain with 11 carbons, which is a new member with the shortest hydrophobic chain among the surfactin family produced by B. subtilis . The critical micelle concentration (CMC) of the surfactin-C11 is 5.59 × 10 −5 mol/L in 10 mmol/L phosphate buffer solution (PBS, pH 7.4) at 25 °C, and the surface tension at CMC ( γ CMC ) is 34.42 mN/m. The hemolytic activities of the surfactin-C11 on 2 % defibrinated sheep blood showed a HC50 of 448.51 μmol/L. Surfactin with short fatty acid chain has low toxicity on erythrocyte, which implies potential applications in biotechnological and pharmaceutical fields.