In vitro and in vivo anticandidal efficacy of green synthesized gold nanoparticles using Spirulina maxima polysaccharide. (May 2020)

• Record Type: Journal Article
• Authors: Dananjaya, S.H.S.; Thu Thao, N.T.; Wijerathna, H.M.S.M.; Lee, Jisoo; Edussuriya, M.; Choi, Dongrack; Saravana Kumar, R.
• Is Part Of: Process biochemistry. Volume 92(2020)
• Page Start: 138
• Page End: 148
• Publication Date: 2020-05
• Subjects: Gold nanoparticles -- Green synthesis -- Spirulina maxima -- Antifungal activity -- Candida albicans -- Biocompatibility; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2020.03.003
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Green synthesis of Au NPs using Spirulina maxima polysaccharides. XRD and UV–vis spectroscopy confirmed the formation of Au NPs. Remarkable in vitro and in vivo antifungal activity of Au NPs against pathogenic Candida albicans. No cytotoxic effect of Au NPs towards HEK293 T and A549 mammalian cells. Abstract: This study, for the first time, demonstrated an unprecedented approach for the green synthesis of gold (Au) nanoparticles (NPs) using the polysaccharide of Spirulina maxima as a reducing agent. Time-kill kinetic analysis was used to evaluate the antifungal activity of the green synthesized Au NPs against the pathogenic Candida albicans ( C. albicans ). The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were found to be 32 μg/mL and 64 μg/mL, respectively. Ultra-structural analysis indicated prominent damage on cell wall of the C. albicans after Au NPs treatment, and suggested that the treatment could increase the membrane permeability and disintegration of cells leading to cellular death. The results of propidium iodide (PI) uptake assay showed the higher level of cell death in Au NPs treated C. albicans cells, further confirming the loss of plasma membrane integrity. Cytotoxicity analysis of Au NPs on HEK293T and A549 cells showed no cytotoxic effect up to 64 μg/mL of Au NPs concentration, indicating the potential use in in vivo studies. Also, the recovery of C. albicans infected zebrafish after Au NPs therapy suggest green synthesized Au NPs from S. maxima polysaccharide as a prospective anticandidal agent.