Mass Spectrometry–Based Analysis of Urinary Biomarkers for Dietary Tomato Intake. Issue 12 (18th June 2020)

• Record Type: Journal Article
• Authors: Hövelmann, Yannick; Lewin, Lea; Steinert, Katharina; Hübner, Florian; Humpf, Hans‐Ulrich
• Is Part Of: Molecular nutrition & food research. Volume 64:Issue 12(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-06-18
• Subjects: alkaloids -- dietary biomarkers -- mass spectrometry -- nutrition -- tomatoes; Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.202000011
• Languages: English
• ISSNs: 1613-4125

Abstract

Abstract : Scope: In this study, the applicability of several β‐carboline, imidazole, and steroidal alkaloids as biomarkers for tomato juice intake is evaluated. Methods and results: Over the course of a 2‐week crossover dietary intervention study, 14 volunteers were given low and high doses of tomato juice after 3 days of avoiding tomato‐based products. On the day of consumption and the following days, volunteers provided urine samples that were quantitatively analyzed by high‐performance liquid chromatography‐tandem mass spectrometry. Herein, glucose‐derived β‐carboline alkaloids are determined as supporting, yet non‐specific dietary biomarkers for tomato juice intake. Several imidazole alkaloids represent further biomarkers, which are shown to specifically indicate consumption of tomato juice for 24 h and partly >24 h. Additionally, steroidal alkaloids derived from esculeogenin B are determined to be specific biomarkers for tomato juice detectable for at least 48 h after consumption. The intake of low and high amounts of tomato juice is significantly distinguishable based on the urinary excretion of all determined biomarkers as well. Conclusions: The dietary intake of tomato juice is conclusively traceable based on urinary excretion of multiple β‐carboline, imidazole, and steroidal alkaloids, and can be determined for up to 48 h after consumption. Furthermore, different intake doses can clearly be distinguished based on their urinary excretion. Abstract : Dietary biomarkers hold great potential for conclusively investigating the relation between food intake and potentially related health effects. The present study reveals several imidazole and steroidal alkaloids to be the first specific biomarkers for the intake of tomato juice. Additionally, β‐carboline alkaloids are determined as supporting, yet non‐specific biomarkers for tomato juice consumption.