Dietary apple polyphenols promote fat browning in high‐fat diet‐induced obese mice through activation of adenosine monophosphate‐activated protein kinase α. (9th February 2020)

• Record Type: Journal Article
• Authors: Zou, Tiande; Wang, Bo; Li, Shuo; Liu, Yue; You, Jinming
• Is Part Of: Journal of the science of food and agriculture. Volume 100:Number 6(2020)
• Page Start: 2389
• Page End: 2398
• Publication Date: 2020-02-09
• Subjects: apple polyphenols -- high‐fat diet -- browning -- AMP‐activated protein kinase (AMPK) -- adaptive thermogenesis; Food -- Periodicals; Agriculture -- Periodicals; 664
• DOI: 10.1002/jsfa.10248
• Languages: English
• ISSNs: 0022-5142

Abstract

Abstract: BACKGROUND: Promoting brown and beige adipogenesis contributes to adaptive thermogenesis, which provides a defense against obesity and related disorders. Apple polyphenols (APs) play a significant role in treating variety of metabolic diseases. This study was conducted to determine the effects of APs on the development of brown and beige adipocytes and thermogenesis and investigate whether these effects are mediated by adenosine monophosphate‐activated protein kinase (AMPK). High‐fat diet (HFD)‐induced obese mice and differentiated 3T3‐L1 adipocytes were subjected to APs treatment. The thermogenic program and associated regulatory factors, and the involvement of AMPK α was assessed. RESULTS: Dietary APs supplementation reduced adiposity and improved insulin sensitivity in HFD‐induced obese mice. Moreover, APs increased the oxygen consumption and heat production and decreased respiratory exchange ratio, which were accompanied by the upregulation of thermogenic genes expression and the activation of AMPK α in brown fat and inguinal white fat. Further, APs treatment directly increased expression of brown adipogenic markers and induced phosphorylation of AMPK α in differentiated 3T3‐L1 adipocytes, whereas the beneficial effects of APs were reversed by AMPK inhibition. CONCLUSION: Our results provide new insights into the function of APs in regulating brown/beige adipogenesis and adaptive thermogenesis and suggest the potential application of APs in the prevention and therapeutics of obesity and associated metabolic diseases. © 2020 Society of Chemical Industry