Isolation and identification of a Bacillus megaterium strain with ochratoxin A removal ability and antifungal activity. (December 2019)

• Record Type: Journal Article
• Authors: Shang, Linlin; Bai, Xuexia; Chen, Chi; Liu, Lijun; Li, Mingyan; Xia, Xiaoshuang; Wang, Yun
• Is Part Of: Food control. Volume 106(2019)
• Publication Date: 2019-12
• Subjects: Ochratoxin A -- Bacillus species -- Aspergillus ochraceus -- Biological control; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2019.106743
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: Ochratoxin A (OTA) is a type of mycotoxin mainly produced by fungus belonging to the genus Aspergillus and Penicillium and plays a critical role in food safety since it causes serious health problem. Biological control by using microorganisms is considered a promising approach for detoxification of this mycotoxin. In order to find bacteria with efficient OTA elimination capability, one bacteria strain JSW-B1 with OTA removal ability was isolated from soil samples and was identified as Bacillus megaterium based on morphological and 16S rRNA analysis. After incubation of isolate JSW-B1 in liquid culture medium containing 2.5 μg/mL OTA for 72 h, the OTA removal percentage was 80.3%. No degradation products of OTA could be detected by liquid chromatography. About 62.6% OTA could be removed by the viable cell fraction after incubation for 72 h compared to 13.7% by cell-free supernatant. Meanwhile, heat treatment significantly enhanced OTA removing capability of the cells, suggesting that the OTA removal activity of the isolate JSW-B1 was mainly attributed to the adsorption by bacterial cells. In addition, the antifungal experiments revealed that isolate JSW-B1 was able to suppress the mycelial growth of Aspergillus ochraceus on solid medium with an inhibition ratio of 41.9% and reduce the fungal incidence on maize corns with a reduction ratio of 15%. Taken together, our results indicated that B. megaterium JSW-B1 could be considered as potential biological agent to control toxigenic fungal growth and mycotoxin contamination in agricultural products. Highlights: B. megaterium JSW-B1 capable of OTA detoxification was isolated and identified. Isolate JSW-B1 inhibits the growth of A. ochraceus on solid medium and maize corn. OTA removal ability is predominately attributed to adsorption by bacterial cells.