Comparison of the production of enzymes to cell wall hydrolysis using different carbon sources by Penicillium echinulatum strains and its hydrolysis potential for lignocelullosic biomass. (March 2018)

• Record Type: Journal Article
• Authors: Schneider, Willian Daniel Hahn; Gonçalves, Thiago Augusto; Uchima, Cristiane Akemi; Reis, Laísa dos; Fontana, Roselei Claudete; Squina, Fabio Marcio; Dillon, Aldo José Pinheiro; Camassola, Marli
• Is Part Of: Process biochemistry. Volume 66(2018)
• Page Start: 162
• Page End: 170
• Publication Date: 2018-03
• Subjects: CAZymes -- Penicillium echinulatum -- Carbon sources -- Enzymatic hydrolysis; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2017.11.004
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Glycerol acts as an inducing source for avicelases. Sugarcane bagasse is an efficient inducer for cellulolytic and hemicellulolytic enzymes. Penicillium echinulatum produces high titers of esterases. The mutant strain produces enzymes with the greatest potential for enzymatic hydrolysis. Enzymes produced using cellulose and glycerol show best hydrolysis performance. Abstract: One of the current challenges to produce second-generation ethanol is to obtain an efficient enzyme complex for hydrolysis of lignocellulosic biomass. Genetic improvement of lignocellulolytic fungi has helped to obtain strains that secrete higher enzymatic titers. This article analyzes the enzymatic profile of two strains of Penicillium echinulatum, wild type 2HH and mutant S1M29, grown on different carbon sources (sugarcane bagasse pretreated by steam explosion, cellulose, glucose, and glycerol) at different cultivation times. To determine enzyme activities, 23 substrates were used. P. echinulatum showed potential to produce cellulases, hemicellulases, esterases, and in minor proportions, pectinases. The mutant strain showed the highest enzymatic titers to most enzymes considered and its enzymatic broth was used in the enzymatic hydrolysis of vegetable biomass. This study broadened the understanding of the enzyme complex of this fungus and the importance of future studies with the mutant strain for use in the production of second-generation ethanol.