Microbial synthesis of undec-9-enoic acid, heptyl ester from renewable fatty acids using recombinant Corynebacterium glutamicum-based whole-cell biocatalyst. (March 2018)

• Record Type: Journal Article
• Authors: Kim, Hyeonsoo; Yang, Jeongmo; Cho, Sukhyeong; Jeong, Kijun; Park, Jinbyung; Lee, Jinwon
• Is Part Of: Process biochemistry. Volume 66(2018)
• Page Start: 61
• Page End: 69
• Publication Date: 2018-03
• Subjects: Biotransformation -- Corynebacterium glutamicum -- Undec-9-enoic acid -- Heptyl ester -- Renewable fatty acid; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2017.12.009
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: C. glutamicum ATCC13032 has high tolerance to n -heptanoic acid. C. glutamicum biocatalyst produces 0.7 mM of undec-9-enoic acid, heptyl ester from renewable fatty acid. This work may help to provide potential of new microbial platform strain for the biotransformation of fatty acid. Abstract: The conversion of ricinoleic acid from renewable sources to long-chain α, ω-dicarboxylic acids or w-hydroxyl carboxylic acids by microbial processes is constrained by toxicity issues. Here, we demonstrate the possible role of Corynebacterium glutamicum as a new microbial strategy for the biotransformation of fatty acids. The established strain Escherichia coli failed to grow at 5 mM n -heptanoic acid, while the specific growth rate of C. glutamicum declined by 28%. We partially constructed a previously designed multistep biocatalytic pathway in C. glutamicum, and confirmed that the C. glutamicum biocatalyst successfully converted ricinoleic acid to undec-9-enoic acid, heptyl ester via 12-keto-oleic acid. We investigated the effects of cultivation and reaction temperatures, and the type and concentration of non-ionic detergent on recombinant C. glutamicum whole-cell bioconversion. At a cultivation temperature of 30 °C and a reaction temperature of 35 °C, and in the presence of 0.09 g/L Triton X-100, the whole-cell C. glutamicum biocatalyst produced 0.8 mM undec-9-enoic acid, heptyl ester from 1.9 mM 12-ketooleic acid. It also generated 0.7 mM undec-9-enoic acid, heptyl ester from 5.5 mM ricinoleic acid. This is the first report of undec-9-enoic acid, heptyl ester production using a recombinant C. glutamicum -based biocatalyst.