Production of recombinant Erns protein of classical swine fever virus and assessment of its enzymatic activity: A recombinant Newcastle disease virus-based approach. (March 2018)

• Record Type: Journal Article
• Authors: Kumar, Rakesh; Kumar, Vishnu; Kumar, Sachin
• Is Part Of: Process biochemistry. Volume 66(2018)
• Page Start: 113
• Page End: 119
• Publication Date: 2018-03
• Subjects: Recombinant -- Newcastle disease virus -- Reverse genetics -- Expression -- RNase; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2017.12.001
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Engineered Newcastle disease virus (NDV) producing E rns protein of classical swine fever virus (CSFV). Characterization of viral and bacterial expressed proteins. Characterization of the recombinant NDV expressing the E rns protein of CSFV. Characterization of the recombinant E rns protein for its RNase activity. Abstract: Classical swine fever virus (CSFV) is an etiological agent of classical swine fever. The structure protein E rns of CSFV assists in the initial attachment of the virus to the host cell and its subsequent entry. Newcastle disease virus (NDV) has been used as a virus vector to express different proteins successfully. The unique characters of NDV lying in its broad host spectrum and ease in production using embryonated chicken egg make it a choice of vector for expressing foreign gene. In the present work, we are reporting the characterization of the ribonuclease (RNase) activity of recombinant NDV expressing E rns of CSFV. The RNase activity of viral expressed recombinant E rns was found to be stable at a optimum temperature of 50 °C. The study will be useful to express and characterize an enzymatic protein in its native form using the NDV as a vector.