Synthesis of core-shell molecularly imprinted polymers (MIP) for spiramycin I and their application in MIP chromatography. (July 2018)

• Record Type: Journal Article
• Authors: Liu, Qi; Wan, Junfen; Cao, Xuejun
• Is Part Of: Process biochemistry. Volume 70(2018)
• Page Start: 168
• Page End: 178
• Publication Date: 2018-07
• Subjects: Spiramycin I -- Core-shell molecularly imprinted polymers -- Selectivity -- Chromatographic column; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2018.04.001
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: The core-shell molecularly imprinted polymers (MIPs) for spiramycin I were polymerized and characterized. The MIPAM showed a fast mass transfer, high adsorption capacity and high selectivity in the static adsorption experiment. The adsorption property of MIPs chromatography were explored. Abstract: Spiramycin I is a main component of spiramycin and a structural analogue of neospiramycin I, which is an impurity in spiramycin production. Molecular imprinting technology (MIT) has many advantages in selectivity and environmental compatibility in biomolecular separation. In this study, core-shell molecularly imprinted polymers (MIPs) were prepared for spiramycin I. Methyl methacrylate (MMA) was used to prepare the seed core, and acrylamide (AM)/methacrylic acid (MAA) was chosen to prepare the shell. The structure of polymerized MIPs was successfully characterized by transmission electron microscopy, dynamic light scattering, Fourier transform infrared spectroscopy and elemental analysis. The adsorption results showed that MIPs polymerized by different monomers exhibited very different adsorption for spiramycin I. MIPs with monomeric AM achieved a fast mass transfer, high adsorption capacity and high selectivity. Then, these MIPs were applied to separate spiramycin I from the fermentation broth in the chromatographic column, and its recovery and purity were 61% and 83%, respectively. Finally, regeneration of the MIP chromatographic column was studied to explore its recycling performance in application.