Lipid production from a mixture of sugarcane top hydrolysate and biodiesel-derived crude glycerol by the oleaginous red yeast, Rhodosporidiobolus fluvialis. (March 2018)

• Record Type: Journal Article
• Authors: Poontawee, Rujiralai; Yongmanitchai, Wichien; Limtong, Savitree
• Is Part Of: Process biochemistry. Volume 66(2018)
• Page Start: 150
• Page End: 161
• Publication Date: 2018-03
• Subjects: Microbial lipid -- Oleaginous yeast -- Sugarcane top hydrolysate -- Biodiesel-derived crude glycerol; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2017.11.020
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Lipid production by oleaginous red yeast, R. fluvialis DMKU-SP314, was optimized. DMKU-SP314 accumulate high lipid in sugarcane top hydrolysate and crude glycerol. Lipid production in a 2 L fermenter achieved high lipid content of 75%. DMKU-SP314 has potential for lipid production from low-cost substrates. Abstract: Lipid was produced by Rhodosporidiobolus fluvialis DMKU-SP314 from a mixture of low-cost substrates: sugarcane top hydrolysate (STH) and biodiesel-derived crude glycerol. The optimized lipid production medium for shaking flask cultivation contained STH supplemented with 59 g/L crude glycerol, 0.21 g/L soybean powder, 0.9 g/L (NH4 )2 SO4, 0.4 g/L KH2 PO4, 2.0 g/L MgSO4 ·7H2 O and pH 6.1 (C/N ratio 60), while the optimal cultivation temperature was 28 °C. Batch cultivation in a 2 L stirred-tank fermenter at an agitation speed of 300 rpm and an aeration rate of 2 vvm achieved the maximum lipid quantity of 18.2 g/L, which was 75.0% of dry biomass (24.3 g/L biomass), after 240 h of cultivation. These represent a 5.4-fold and 1.7-fold improvement in the lipid concentration and lipid content, respectively, compared to non-optimized shaking flask cultivation. Under optimal conditions, the lipid produced had a high monounsaturated fatty acid content (36.6% of oleic acid), which is suitable for biodiesel production. Thus, R. fluvialis DMKU-SP314 is capable of producing large quantities of lipid from low-cost raw materials in a process that can be readily scaled up for industrial production.