Enzymatic synthesis of d-alanine from a renewable starting material by co-immobilized dehydrogenases. (March 2018)

• Record Type: Journal Article
• Authors: Li, Jianjiong; Chen, Xi; Cui, Yunfeng; Liu, Weidong; Feng, Jinhui; Wu, Qiaqing; Zhu, Dunming
• Is Part Of: Process biochemistry. Volume 66(2018)
• Page Start: 126
• Page End: 132
• Publication Date: 2018-03
• Subjects: d-alanine -- Co-immobilized enzymes -- Meso-diaminopimelate dehydrogenase -- Formate dehydrogenase; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2017.12.008
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: A green method was developed for the preparation of optically pure d -alanine from sodium pyruvate. A meso -diaminopimelate dehydrogenase and formate dehydrogenase were co-immobilized on a resin. The co-immobilized enzymes remained 80% of the initial activity after 12 reuses. d -alanine was obtained with 75.7% isolation yield and >99.5% ee for the first 20 batch reactions. Abstract: d -alanine is an important pharmaceutical intermediate. In this study, an effective method was developed to prepare optically pure d -alanine from sodium pyruvate with co-immobilized meso- diaminopimelate dehydrogenase from Symbiobacterium thermophilum and formate dehydrogenase from Candida Boidinii onto a resin. After optimizing the conditions of immobilization and biotransformation, the co-immobilized enzymes exhibited excellent stereoselectivity, yield and reusability. The co-immobilized enzymes kept 80% of its activity after 12 reuses, and d -alanine was isolated in 75.7% average yield and 99.5% ee for the first 20 cycles in the batch reactions. This suggests the co-immobilized enzyme system as a promising biocatalyst for the green production of d -alanine from pyruvic acid, a bio-based starting material.