Tuna Bone Powder Alleviates Glucocorticoid‐Induced Osteoporosis via Coregulation of the NF‐κB and Wnt/β‐Catenin Signaling Pathways and Modulation of Gut Microbiota Composition and Metabolism. Issue 5 (29th January 2020)

• Record Type: Journal Article
• Authors: Li, Jingjing; Yang, Mingyuan; Lu, Chenyang; Han, Jiaojiao; Tang, Shasha; Zhou, Jun; Li, Ye; Ming, Tinghong; Wang, Zaijie Jim; Su, Xiurong
• Is Part Of: Molecular nutrition & food research. Volume 64:Issue 5(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-01-29
• Subjects: glucocorticoid‐induced osteoporosis -- gut microbiota -- short‐chain fatty acids -- signaling pathways -- tuna bone powder; Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.201900861
• Languages: English
• ISSNs: 1613-4125

Abstract

Abstract : Scope: The effects and mechanism of tuna bone powder (TBP) on glucocorticoid‐induced osteoporosis (GIOP) alleviation in terms of signaling pathway coregulation and gut microbiota modulation are investigated. Methods and results: The powder size distribution and composition of TBP are measured. The GIOP female mice induced by dexamethasone intramuscular injection are used to examine the anti‐osteoporosis effects of TBP in a 10 week experiment, and improved bone mineral density and bone microarchitecture are observed via micro‐CT. In addition, qRT‐PCR results show that the NF‐κB pathway is inhibited to reduce bone resorption, whereas the Wnt/β‐catenin pathway is activated to enhance bone formation after treatment. Moreover, TBP treatments suppress the release of pro‐inflammatory cytokines, repair dysfunction of the intestinal epithelial barrier, and prevent aggravated systemic inflammation in mRNA levels. Additionally, 16S rRNA gene sequencing indicate that TBP treatments enhance the abundance of anti‐inflammatory bacteria and short‐chain fatty acid (SCFA) producers, which is consistent with increased SCFA contents in feces measured via GC–MS. Conclusion: These data show that TBP ameliorates GIOP in mice through four aspects, including coregulating signaling pathways, blocking proinflammatory cytokines, repairing the intestinal epithelial barrier, and modulating gut microbiota. Therefore, TBP may be a potential prebiotic agent to alleviate osteoporosis in humans. Abstract : Glucocorticoid‐induced osteoporosis (GIOP) in mice is dose‐dependently ameliorated by tuna bone powder treatments through four aspects, including coregulating NF‐κB and Wnt/β‐catenin signaling pathways to inhibit bone resorption and activate bone formation, blocking proinflammatory cytokines (TNF‐α, IL‐1, IL‐6, and IL‐17), repairing the intestinal epithelial barrier (Occludin, ZO‐1, and Claudin‐1), and modulating gut microbiota composition and its derived short‐chain fatty acids productions.