Regulation of trehalose, a typical stress protectant, on central metabolisms, cell growth and division of Saccharomyces cerevisiae CEN.PK113-7D. (August 2020)

• Record Type: Journal Article
• Authors: Zhang, Xiaoru; Zhang, Yaxian; Li, Hao
• Is Part Of: Food microbiology. Volume 89(2020)
• Publication Date: 2020-08
• Subjects: Trehalose -- Saccharomyces cerevisia CEN.PK113-7D -- Cell growth -- Metabolisms -- Cell cycle; ACT1 actin -- ATH1 acid trehalase -- APT6 F1Fo ATP synthase subunit a -- APT8 F1Fo ATP synthase subunit 8 -- CDK1 cyclin-dependent kinase -- CIT1 citrate-synthase -- HXK2 hexokinase 2 -- HXT hexose transporter -- IDH1 isocitrate dehydrogenase -- KGD1 alpha-ketoglutarate dehydrogenase -- NDE1 NADH dehydrogenase -- NDE2 NADH dehydrogenase -- NDI1 NADH dehydrogenase -- NTH1 neutral trehalase -- PFK1 phosphofructokinase-1 -- PYK1 pyruvate kinase -- TPS1 trehalose-phosphate synthase -- TPS2 trehalose-phosphatase -- ZWF1 glucose-6-phosphate dehydrogenase; Food Microbiology -- Periodicals; Aliments -- Microbiologie -- Périodiques; Food -- Microbiology; Periodicals; Food -- Microbiology -- Periodicals; Food contamination -- Periodicals; 664.001579
• DOI: 10.1016/j.fm.2020.103459
• Languages: English
• ISSNs: 0740-0020

Abstract

Abstract: Trehalose could protect the typical food microorganism Saccharomyces cerevisiae cell against environmental stresses; however, the other regulation effects of trehalose on yeast cells during the fermentation are still poorly understood. In this manuscript, different concentrations (i.e., 0, 2 and 5% g/v) of trehalose were respectively added into the medium to evaluate the effect of trehalose on growth, central metabolisms and division of S. cerevisiae CEN.PK113-7D strain that could uptake exogenous trehalose. Results indicated that addition of trehalose could inhibit yeast cell growth in the presence or absence of 8% v/v ethanol stress. Exogenous trehalose inhibited the glucose transporting efficiency and reduced intracellular glucose content. Simultaneously, increased intracellular trehalose content destroyed the steady state of trehalose cycle and caused the imbalance between the upper glycolysis part and the lower part, thereby leading to the dysfunction of glycolysis and further inhibiting the normal yeast cell growth. Moreover, energy metabolisms were impaired and the ATP production was reduced by addition of trehalose. Finally, exogenous trehalose-associated inhibition on yeast cell growth and metabolisms delayed cell cycle. These results also highlighted our knowledge about relationship between trehalose and growth, metabolisms and division of S. cerevisiae cells during fermentation. Highlights: Exogenous trehalose could inhibit S. cerevisiae CEN.PK113-7D cell growth. Trehalose inhibited the glucose transporting efficiency of S. cerevisiae cells. Trehalose caused imbalance between upper glycolysis part and lower part. Exogenous trehalose impaired the energy metabolisms of S. cerevisiae cells. Exogenous trehalose could delay yeast cell cycle.