Effects and blood concentrations of cobalt after ingestion of 1 mg/d by human volunteers for 90 d. Issue 3 (5th February 2014)

• Record Type: Journal Article
• Authors: Tvermoes, Brooke E; Unice, Kenneth M; Paustenbach, Dennis J; Finley, Brent L; Otani, Joanne M; Galbraith, David A
• Is Part Of: American journal of clinical nutrition. Volume 99:Issue 3(2014)
• Page Start: 632
• Page End: 646
• Publication Date: 2014-02-05
• Subjects: Diet therapy -- Periodicals; Nutrition -- Periodicals; Dietetics -- Periodicals; 613.205
• DOI: 10.3945/ajcn.113.071449
• Languages: English
• ISSNs: 0002-9165

Abstract

ABSTRACT: Background: Over-the-counter cobalt supplements are available for sale in the United States, but little is known regarding their clinical effects and biokinetic distribution with long-term use. Objective: We assessed blood kinetics, biochemical responses, and clinical effects in 5 adult men and 5 adult women who voluntarily ingested ~1.0 mg Co/d (0.080–0.19 mg Co · kg −1 · d −1 ) of a commercially available cobalt supplement over a 3-mo period. Design: Volunteers were instructed to take the cobalt dietary supplement in the morning according to the manufacturer's label. Blood samples were collected and analyzed for a number of biochemical variables before, during, and after dosing. Hearing, vision, cardiac, and neurologic functions were also assessed in volunteers before, during, and after dosing. Results : After ~90 d of dosing, mean cobalt blood concentrations were lower in men than in women. Mean cobalt whole blood and serum concentrations in men were 20 μg/L (range: 12–33 μg/L) and 25 μg/L (range: 15–46 μg/L), respectively. In women, mean cobalt whole blood and serum concentrations were 53 μg/L (range: 6–117 μg/L) and 71 μg/L (range: 9–149 μg/L), respectively. Estimated red blood cell (RBC) cobalt concentrations suggested that cobalt was sequestered in RBCs during their 120-d life span, which resulted in a slower whole blood clearance compared with serum. The renal clearance of cobalt increased with the serum concentration and was, on average, lower in women (3.5 ± 1.3 mL/min) than in men (5.5 ± 1.9 mL/min). Sex-specific differences were observed in cobalt absorption and excretion. There were no clinically significant changes in biochemical, hematologic, and clinical variables assessed in this study. Conclusion: Peak cobalt whole blood concentrations ranging between 9.4 and 117 μg/L were not associated with clinically significant changes in basic hematologic and clinical variables. This study was registered at clinicaltrials.gov as NCT01990794.