Online monitoring of enzymatic hydrolysis of marine by-products using benchtop nuclear magnetic resonance spectroscopy. (June 2020)

• Record Type: Journal Article
• Authors: Anderssen, Kathryn E.; McCarney, Evan R.
• Is Part Of: Food control. Volume 112(2020)
• Publication Date: 2020-06
• Subjects: Enzymatic hydrolysis -- Nuclear magnetic resonance spectroscopy -- Process monitoring -- Marine byproducts; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2019.107053
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: Enzymatic hydrolysis is becoming a more commonly used method to create high value products from traditionally low value marine by-products. However, improvement to processing is hampered by a lack of ways to characterize the reaction in real time. Current methods of analysis rely on taking offline samples, deactivating the enzymes, and performing analysis on the products afterwards. Nuclear magnetic resonance benchtop spectroscopy was investigated as a method for online process monitoring of enzymatic hydrolysis. Online and offline NMR measurements were performed for enzymatic hydrolysis reactions on red cod, salmon and shrimp. Both the online and offline measurements were able to follow the reaction process and showed good agreement in their calculated reaction rate. Application of the methodology to several types of raw materials indicates the technique is robust with regards to sample type. Advantages and disadvantages of low-field versus high-field NMR spectroscopy are discussed as well as practical considerations needed in order to apply the method industrially. Highlights: Benchtop NMR spectroscopy evaluated for monitoring enzymatic hydrolysis. Able to track reaction progress with good precision. Provides measure of solubilized protein in water phase. Good agreement between calculated online and offline reaction rates.