Analysis of bacterial and fungal communities by Illumina MiSeq platforms and characterization of Aspergillus cristatus in Fuzhuan brick tea. (August 2019)

• Record Type: Journal Article
• Authors: Rui, Ying; Wan, Peng; Chen, Guijie; Xie, Minhao; Sun, Yi; Zeng, Xiaoxiong; Liu, Zhonghua
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 110(2019)
• Page Start: 168
• Page End: 174
• Publication Date: 2019-08
• Subjects: Fuzhuan brick tea -- Illumina MiSeq sequencing -- Microbial community -- Fungi -- Aspergillus; Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2019.04.092
• Languages: English
• ISSNs: 0023-6438

Abstract

Abstract: Fuzhuan brick tea (FBT) is an indigenous dark tea fermented by fungal and bacterial communities, and the special favor of FBT attributes to the actions of microbiota, while the correlation between bacteria and fungi attracts little attention. In the present study, therefore, the compositions of microbial communities in 10 samples of FBT from four tea processing factories were evaluated by Illumina MiSeq sequencing. Bacterial taxonomy analysis revealed that the phylum Firmicutes and Proteobacteria were the predominant bacteria in all samples. Fungal taxonomy analysis revealed that the phylum Ascomycota, family Trichocomaceae, genus Aspergillus was the dominant fungus in all samples. Moreover, Aspergillus (OTU1, OTU2, OTU16, OTU30, OTU31 and OTU37), Wallemia (OTU3), Penicillium (OTU32), Candida (OTU33, OTU35, OTU40 and OTU43), Debaryomyces (OTU48) and Rhodotorula (OTU68) showed higher correlation with bacteria in FBT. Furthermore, a strain Aspergillus cristatus was isolated from FBT, and its morphology and molecular taxonomy were observed and analyzed by scanning electron microscopy and sequencing. Hence, this is the first report to reveal the correlation between bacterial and fungal communities in FBT, and A. cristatus may have potential application to improve the quality of FBT. Highlights: Bacterial and fungal communities of Fuzhuan brick tea (FBT) were investigated. Phylum Ascomycota, family Trichocomaceae, genus Aspergillus, dominant fungus in FBT. OTU1 ( Aspergillus ) had negative correlations with 7 and postive with 2 bacteria. Aspergillus cristatus was isolated from FBT and identified by SEM and ITS sequencing.