Egg White–Derived Antihypertensive Peptide IRW (Ile‐Arg‐Trp) Reduces Blood Pressure in Spontaneously Hypertensive Rats via the ACE2/Ang (1‐7)/Mas Receptor Axis. Issue 9 (10th April 2019)

• Record Type: Journal Article
• Authors: Liao, Wang; Fan, Hongbing; Davidge, Sandra T.; Wu, Jianping
• Is Part Of: Molecular nutrition & food research. Volume 63:Issue 9(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-04-10
• Subjects: ACE2 -- bioactive peptides -- blood pressure -- hypertension; Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.201900063
• Languages: English
• ISSNs: 1613-4125

Abstract

Abstract : Scope: It is found in the previous study that egg‐white‐derived antihypertensive peptide Ile‐Arg‐Trp (IRW) upregulated angiotensin converting enzyme 2 (ACE2) in spontaneously hypertensive rats (SHRs). The objective of this study is to evaluate the contribution of ACE2 activation by IRW to blood‐pressure‐lowering activity in vivo. Methods and results: Adult male SHRs (13–15 week old) are assigned into four groups: 1) untreated with saline infusion; 2) IRW administration (15 mg per kg body weight) with saline infusion; 3) Mas receptor (MasR) antagonist A779 (48 µg per kg body weight per h) infusion; 4) A779 infusion and IRW. Animals are implanted with telemetry transmitter first, and then an osmotic pump filled with saline or A779 is implanted. A779/saline is infused for 7 days, continued with an additional 7 days of treatments. Results indicate that blocking MasR abolished the blood‐pressure‐lowering effect of IRW. Akt/eNOS signaling in aorta is upregulated by IRW treatment but deactivated by A779 infusion. Circulating levels of interleukin 6 and monocyte chemoattractant protein 1, along with cyclooxygenase 2 in aorta are reduced by IRW but restored by A779 infusion. Conclusion: IRW reduces blood pressure of SHR via the ACE2/Ang (1‐7)/MasR axis. Mechanisms pertaining to IRW as an ACE2 activator in vivo include enhanced endothelium‐dependent vasorelaxation and reduced vascular inflammation. Abstract : ACE2/Ang (1‐7)/MasR axis is activated by an egg‐white‐ovotransferrin‐derived antihypertensive peptide IRW (Ile‐Arg‐Trp), which is responsible for lowering blood pressure in spontaneously hypertensive rats. This is the first report demonstrating the predominant role of the ACE2/Ang (1‐7)/MasR axis in having the blood‐pressuring‐lowering effect of antihypertensive peptide. This study highlights that the ACE2/Ang (1‐7)/MasR axis can be a new target for identifying novel antihypertensive peptides from food protein sources.