Anticholinergic effects of Actinidia arguta fruits and their polyphenol content determined by liquid chromatography-photodiode array detector-quadrupole/time of flight-mass spectrometry (LC-MS-PDA-Q/TOF). (15th January 2019)

• Record Type: Journal Article
• Authors: Wojdyło, Aneta; Nowicka, Paulina
• Is Part Of: Food chemistry. Volume 271(2019)
• Page Start: 216
• Page End: 223
• Publication Date: 2019-01-15
• Subjects: LC-MS-PDA-Q/TOF -- Flavonols -- Flavanols -- Phenolic acid -- Anthocyanin -- AChE -- BuChE -- Inhibition; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2018.07.084
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Actinidia arguta is a promising new crop well adapted to European weather conditions. A total of 31 polyphenolic compounds were identified in fruits by LC-MS-PDA-Q/TOF method. Mainly glycosylated and acetylated forms of quercetin and kaempferol were present in A. arguta . Anticholinergic effect of A. arguta fruits can be depend on phenolic compounds, especially flavonols. A. arguta fruits showed strong nutritional potential to improve human health. Abstract: This study discusses polyphenolic compounds identified and quantified in Actinidia arguta fruits by LC-MS-PDA-Q/TOF method and in vitro anticholinergic activity. Notably, of 31 compounds, including 16 flavonols, 7 flavanols, 7 phenolic acids, and 1 anthocyanin were identified or tentatively identified on the basis of their retention times, accurate mass measurements and subsequent mass fragmentation data, or by comparison with reference substances and literature. Among the detected compounds, 27 were reported for the first time in A. arguta fruits. The content of total polyphenols equal 845.54 mg/100 g dry weight (dw), and flavanols predominat (92% of total phenolic compounds). Flavonol derivatives, mainly glycosylated and acetylated forms of quercetin (22.64 mg/100 g dw) and kaempferol (18.40 mg/100 g dw) were quantified. The total content of phenolic acids was 29.63 mg/100 g dw, and neochlorogenic acid predominant. This anticholinergic activity effect of A. arguta fruits can be explained by the Pearson's correlation found between flavonols (r = 0.709 and 0.678), phenolic acids (r = 0.513 and 0.487), flavan-3-ols (r = 0.466 and 0.443) and anthocyanins (r = 0.312 and 0.301) for acetylcholinesterase (AChE) or butylcholinoesterase (BuChE), respectively. The data compiled from the quantitative polyphenol indicate that A. arguta fruits could be regarded as a promising source of bioactive functional food.