Lipase-catalyzed glycerolysis of anchovy oil in a solvent-free system: Simultaneous optimization of monoacylglycerol synthesis and end-product oxidative stability. (15th January 2019)

• Record Type: Journal Article
• Authors: Palacios, David; Ortega, Natividad; Rubio-Rodríguez, Nuria; Busto, María D.
• Is Part Of: Food chemistry. Volume 271(2019)
• Page Start: 372
• Page End: 379
• Publication Date: 2019-01-15
• Subjects: Lipase PS-DI -- Monoacylglycerols (MAG) -- Oxidative stability -- Polyunsaturated fatty acids (PUFA) -- Solvent-free glycerolysis; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2018.07.184
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Solvent-free glycerolysis catalyzed by Lipase PS-DI produces MAG rich in PUFA. For the first time, oxidative stability is considered during glycerolysis design. Simultaneous optimization of MAG yield and oxidative stability in glycerolysis design. Optimized glycerolysis conditions improved end-product oxidative stability by 68%. Abstract: The production of mono- and diacylglycerols rich in polyunsaturated fatty acids is achieved in this study, by solvent-free glycerolysis of anchovy oil with lipase PS-DI from Burkholderia cepacia . Attention is focused on the oxidative stability of the reaction products, determined in terms of induction time (It ). The effects of glycerol/triacylglycerol molar ratio, enzyme concentration, and reaction temperature on mono- and diacylglycerol production and It are all assessed. The operating conditions that optimized monoacylglycerol yields and oxidative stability were a glycerol/triacylglycerol ratio of 3/1, 9.0% (w/w) Lipase PS-DI, a stirring rate of 200 rpm, and a reaction time of 4 h, at 45.8 °C, producing a content of 24.8% and 51.9% of mono- and diacylglycerols, respectively, over an It of 1.41 h. The glycerolysis conditions determined by simultaneous optimization strategy increased the oxidative stability of the glycerolysis products by 68%, which rose from 0.84 h (individual optimization) to 1.41 h.